Table 1. Oligonucleotide Primers Used in... | American Society of Hematology

Table 1.

Oligonucleotide Primers Used in Sequencing theGLCLC Gene

	Oligo Sequence 5′ to 3′	Region Amplified by PCR	Fragment Size (bp)
Sense	TCATGTACTCACTCATTTAGC	Promoter putative antioxidant responsive element	243
Antisense	CTAGAGTTGGCCTTATCTCG
Sense	CGCGTTTGCGTAAAGCGAGG	Promoter TATA and CAAT boxes	331
Antisense	GGAGCATGCCCAGTCTTTGC
Sense	GCTGAGTGTCCGTCTCGCG^*	cDNA −77 to 684	761
Antisense	TGAAGCTTCATCATCCTCAG^*
Sense	GCAATAAACAAGCACCCTCG^*	cDNA 535 to 1183	649
Antisense	GACTCATTAGCATCATCCAGG^*
Sense	TATAATGACATCGACTTGACG^*	cDNA 1028 to 1570	543
Antisense	TTCCCATTGATGATGGTGTC^*
Sense	AGGCCCAGAACAGCACGGAG^*	cDNA 1527 to 2044	518
Antisense	TCAATTTCTGGCTCACTGGC^*
Sense	ACTGACTCATCCAACTAGAC	cDNA 1896 to 2044 for 3′ polymorphic insertion	149 or 153
Antisense	TCAATTTCTGGCTCACTGGC
Sense	CCTCCAAACTCAGACATTGG	Genomic fragment containing G/A intron polymorphism	397
Antisense	CAAACACCACATAGGCAGAG

	Oligo Sequence 5′ to 3′	Region Amplified by PCR	Fragment Size (bp)
Sense	TCATGTACTCACTCATTTAGC	Promoter putative antioxidant responsive element	243
Antisense	CTAGAGTTGGCCTTATCTCG
Sense	CGCGTTTGCGTAAAGCGAGG	Promoter TATA and CAAT boxes	331
Antisense	GGAGCATGCCCAGTCTTTGC
Sense	GCTGAGTGTCCGTCTCGCG^*	cDNA −77 to 684	761
Antisense	TGAAGCTTCATCATCCTCAG^*
Sense	GCAATAAACAAGCACCCTCG^*	cDNA 535 to 1183	649
Antisense	GACTCATTAGCATCATCCAGG^*
Sense	TATAATGACATCGACTTGACG^*	cDNA 1028 to 1570	543
Antisense	TTCCCATTGATGATGGTGTC^*
Sense	AGGCCCAGAACAGCACGGAG^*	cDNA 1527 to 2044	518
Antisense	TCAATTTCTGGCTCACTGGC^*
Sense	ACTGACTCATCCAACTAGAC	cDNA 1896 to 2044 for 3′ polymorphic insertion	149 or 153
Antisense	TCAATTTCTGGCTCACTGGC
Sense	CCTCCAAACTCAGACATTGG	Genomic fragment containing G/A intron polymorphism	397
Antisense	CAAACACCACATAGGCAGAG

Oligonucleotides used for amplification. Some additional nested oligonucleotides were used for sequencing.