Primers Used for Single Copy PCR of the IκB Gene
| . | . | Sequence . |
|---|---|---|
| I | Sense (5′ → 3′): | AGA AAA GGC ACT GAC CAT GG |
| (pos 2283-2303) | ||
| Antisense (5′ → 3′): | GGC ATC CAA TAG GCA CTT TG (pos 3058-3039) | |
| Antisense (reamplification) (5′ → 3′): | GGA GTT TAA GCT CTT GCC TGG (pos 3007-2987) | |
| II | Sense (5′ → 3′): | ATG GTA TGT CTG CCT CCC TG (pos 2902-2921) |
| Antisense (5′ → 3′): | CCT CAT TAG TTA GAG CGC CG (pos 3699-3680) | |
| Antisense (reamplification) (5′ → 3′): | AGG TGG GCC AAC TTA AGG AGT C (pos 2966-2945) | |
| III | Sense (5′ → 3′): | CCA TTC CCT TCA CCC TCC (pos 3702-3719) |
| Antisense (5′ → 3′): | ACC ACT GGG GTC AGT CAC TC (pos 4391-4372) | |
| Antisense (reamplification) (5′ → 3′): | AGG CCC CTA GAG CTG CTC (pos 3712-3695) |
| . | . | Sequence . |
|---|---|---|
| I | Sense (5′ → 3′): | AGA AAA GGC ACT GAC CAT GG |
| (pos 2283-2303) | ||
| Antisense (5′ → 3′): | GGC ATC CAA TAG GCA CTT TG (pos 3058-3039) | |
| Antisense (reamplification) (5′ → 3′): | GGA GTT TAA GCT CTT GCC TGG (pos 3007-2987) | |
| II | Sense (5′ → 3′): | ATG GTA TGT CTG CCT CCC TG (pos 2902-2921) |
| Antisense (5′ → 3′): | CCT CAT TAG TTA GAG CGC CG (pos 3699-3680) | |
| Antisense (reamplification) (5′ → 3′): | AGG TGG GCC AAC TTA AGG AGT C (pos 2966-2945) | |
| III | Sense (5′ → 3′): | CCA TTC CCT TCA CCC TCC (pos 3702-3719) |
| Antisense (5′ → 3′): | ACC ACT GGG GTC AGT CAC TC (pos 4391-4372) | |
| Antisense (reamplification) (5′ → 3′): | AGG CCC CTA GAG CTG CTC (pos 3712-3695) |