The Initial Rate of EPO Internalization and Extent of Bound EPO Internalized for 32D Cell Lines Expressing EPO-R Variants
| EPO-R Variant . | Internalization Rate (%/min) . | EPO Internalized at 40 Minutes (% initially bound) . |
|---|---|---|
| 1-483 (wild-type) | 3.2 | 54 |
| 1-411 | 3.6 | 50 |
| 1-373 | 4.2 | 58 |
| 1-321 | 3.8 | 55 |
| 1-252 | 1.0 | 20 |
| 1-483(W282R) | 3.0 | 52 |
| 1-483(YF) | 2.9 | 45 |
| EPO-R Variant . | Internalization Rate (%/min) . | EPO Internalized at 40 Minutes (% initially bound) . |
|---|---|---|
| 1-483 (wild-type) | 3.2 | 54 |
| 1-411 | 3.6 | 50 |
| 1-373 | 4.2 | 58 |
| 1-321 | 3.8 | 55 |
| 1-252 | 1.0 | 20 |
| 1-483(W282R) | 3.0 | 52 |
| 1-483(YF) | 2.9 | 45 |
The initial rate of internalization was determined from the slope of the linear portion of the curve between 0 and 10 minutes.