Summary of the Clonal Analyses of Primary and Secondary Recipients of neo- or HOXB4-Transduced Cells Presented in Fig 4, Demonstrating Enhanced Polyclonal Long-Term Repopulation byHOXB4-Transduced Stem Cells
| Mice . | Estimated Numbers of Transduced CRU Injected/ 1° Mouse3-150 . | No. of Transduced Clones in 1° Mice, Detected in 2° Mice . | Minimal Life- Span of Each Transduced Clone (wks)3-151 . |
|---|---|---|---|
| neo-32 | 26 | 4 | 48 |
| HOXB4-32 | 16 | 13 | 48 |
| HOXB4-52 | 20 | 15 | 68 |
| Mice . | Estimated Numbers of Transduced CRU Injected/ 1° Mouse3-150 . | No. of Transduced Clones in 1° Mice, Detected in 2° Mice . | Minimal Life- Span of Each Transduced Clone (wks)3-151 . |
|---|---|---|---|
| neo-32 | 26 | 4 | 48 |
| HOXB4-32 | 16 | 13 | 48 |
| HOXB4-52 | 20 | 15 | 68 |
The number of transduced CRU injected per mouse were estimated based on our previous results that determined the frequency of CRU at 1 in 6,000 bone marrow cells as harvested from identical retroviral infection cultures as used in this study8 and the estimation of gene transfer to CRU equal to that of CFC presented in Fig 1B.
The minimal life-span of each clone detected is the sum of the life-span, after transplantation, of primary (32 weeks for theneo mouse and 32 and 52 weeks for the 2 HOXB4 mice) and secondary recipients (16 weeks for all mice).