Interactions of Platelets With THP-1 Cells Are Increased by Passage Through the Flow Chamber
| . | Prechamber . | Coverslip . | Postchamber . | |||
|---|---|---|---|---|---|---|
| Control . | Thromb/6 . | Control . | Thromb/6 . | Control . | Thromb/6 . | |
| THP-1 | 475 | 413 | 452 | 455 | 446 | 388 |
| THP-1 + platelet | 160* | 70† | 472 | 29† | 318‡ | 70† |
| Total | 635 | 483 | 924 | 464 | 764 | 458 |
| . | Prechamber . | Coverslip . | Postchamber . | |||
|---|---|---|---|---|---|---|
| Control . | Thromb/6 . | Control . | Thromb/6 . | Control . | Thromb/6 . | |
| THP-1 | 475 | 413 | 452 | 455 | 446 | 388 |
| THP-1 + platelet | 160* | 70† | 472 | 29† | 318‡ | 70† |
| Total | 635 | 483 | 924 | 464 | 764 | 458 |
Recruitment of platelet-decorated THP-1 cells to HUVEC is favored over platelet-free THP-1 cells. P-selectin monoclonal antibodies inhibit platelet/THP-1 interaction in suspension in response to shear stress as well as adhesion of THP-1/platelet complexes to HUVEC. Data represent individual cell counts from at least 5 independent experiments.
P < .0001 prechamber v postchamber and coverslip.
P < .001 Thromb/6 v control.
P < .001 v coverslip.