Molecular Masses of Achromobacter Protease I-Digested Peptides From the 68- and 70-kD Btk-SH2 Domain-Binding Proteins
| Observed Mass (daltons) . | Theoretical Mass (daltons)* . | Δ† . | Sequences From Data Base . |
|---|---|---|---|
| 68-kD Protein | |||
| 785.6 | 785.5 | 0.1 | LRQLQK |
| 835.6 | 835.5 | 0.1 | QYALGRK |
| 935.7 | 935.5 | 0.2 | DGSFLIRK |
| 1105.7 | 1105.5 | 0.2 | GRNSGAWETK1-153 |
| 1224.8 | 1224.5 | 0.3 | PWYAGACDRK |
| 1241.8 | 1241.6 | 0.2 | SAWWALHRSNK |
| 1356 | 1355.7 | 0.3 | QPYTLVVFFNK‡ |
| 1443.1 | 1442.8 | 0.3 | SPPPAAPSPLPRAGK |
| 1584.2 | 1583.9 | 0.3 | ARLTSTLPALTALQK |
| 1706.3 | 1706 | 0.3 | RVYNIPVRFIEATK‡ |
| 3007.2 | 3006.6 | 0.6 | PIPAERHRGSSHRQEAVQSPVFPPAQK |
| 70-kD Protein | |||
| 785.6 | 785.5 | 0.1 | LRQLQK |
| 835.6 | 835.5 | 0.1 | QYALGRK |
| 935.6 | 935.5 | 0.1 | DGSFLIRK |
| 1224.7 | 1224.5 | 0.2 | PWYAGACDRK |
| 1241.7 | 1241.6 | 0.1 | SAEEALHRSNK |
| 1355.9 | 1355.7 | 0.2 | QPYTLVVFFNK‡ |
| 1443 | 1442.8 | 0.2 | SPPPAAPSPLPRAGK |
| 1584.2 | 1583.9 | 0.3 | ARLTSTLPALTALQK |
| 1706.2 | 1706 | 0.2 | RVYNIPVRFIEATK‡ |
| 2357.4 | 2357.1 | 0.3 | PNSSTPASPPGTASGRNSGAWETK1-155 |
| 3007.1 | 3006.6 | 0.5 | PIPAERHRGSSHRQEAVQSPVFPPAQK |
| Observed Mass (daltons) . | Theoretical Mass (daltons)* . | Δ† . | Sequences From Data Base . |
|---|---|---|---|
| 68-kD Protein | |||
| 785.6 | 785.5 | 0.1 | LRQLQK |
| 835.6 | 835.5 | 0.1 | QYALGRK |
| 935.7 | 935.5 | 0.2 | DGSFLIRK |
| 1105.7 | 1105.5 | 0.2 | GRNSGAWETK1-153 |
| 1224.8 | 1224.5 | 0.3 | PWYAGACDRK |
| 1241.8 | 1241.6 | 0.2 | SAWWALHRSNK |
| 1356 | 1355.7 | 0.3 | QPYTLVVFFNK‡ |
| 1443.1 | 1442.8 | 0.3 | SPPPAAPSPLPRAGK |
| 1584.2 | 1583.9 | 0.3 | ARLTSTLPALTALQK |
| 1706.3 | 1706 | 0.3 | RVYNIPVRFIEATK‡ |
| 3007.2 | 3006.6 | 0.6 | PIPAERHRGSSHRQEAVQSPVFPPAQK |
| 70-kD Protein | |||
| 785.6 | 785.5 | 0.1 | LRQLQK |
| 835.6 | 835.5 | 0.1 | QYALGRK |
| 935.6 | 935.5 | 0.1 | DGSFLIRK |
| 1224.7 | 1224.5 | 0.2 | PWYAGACDRK |
| 1241.7 | 1241.6 | 0.1 | SAEEALHRSNK |
| 1355.9 | 1355.7 | 0.2 | QPYTLVVFFNK‡ |
| 1443 | 1442.8 | 0.2 | SPPPAAPSPLPRAGK |
| 1584.2 | 1583.9 | 0.3 | ARLTSTLPALTALQK |
| 1706.2 | 1706 | 0.2 | RVYNIPVRFIEATK‡ |
| 2357.4 | 2357.1 | 0.3 | PNSSTPASPPGTASGRNSGAWETK1-155 |
| 3007.1 | 3006.6 | 0.5 | PIPAERHRGSSHRQEAVQSPVFPPAQK |
Calculated from Achromobacter protease I-digested peptides of human BLNK-s and BLNK whose sequences were deposited in the database.
The difference between observed and theoretical masses.
These peptide sequences were determined by protein microsequencing, which completely coincided with those obtained from sequence database.
Specific for the 68-kD protein (human BLNK-s).
Specific for the 70-kD protein (human BLNK).