Absolute Numbers of B Cells Harvested From Reaggregate Cultures
| FL Cells Transduced With . | % GFP+ Cells . | No. of Recovered GFP+ Cells Per Input of 10,000 GFP+ Cells . | ||
|---|---|---|---|---|
| Before Culture . | After Culture . | Total . | CD10+CD19+ Cells . | |
| IRES-GFP | 28 | 12 | 43,000 | 31,000 |
| Id3-IRES-GFP | 30 | 1 | 6,000 | 1,680 |
| FL Cells Transduced With . | % GFP+ Cells . | No. of Recovered GFP+ Cells Per Input of 10,000 GFP+ Cells . | ||
|---|---|---|---|---|
| Before Culture . | After Culture . | Total . | CD10+CD19+ Cells . | |
| IRES-GFP | 28 | 12 | 43,000 | 31,000 |
| Id3-IRES-GFP | 30 | 1 | 6,000 | 1,680 |
CD34+CD38−CD10− FL cells were sorted and transduced with the IRES-GFP or Id3-IRES-GFP containing virus. Two days later, the percentage of GFP+cells was determined to get the percentage before culture. The cells were reaggregated with the SV19 human stromal cell line for 3 weeks. After the culture period, cells were harvested and analyzed for cell surface expression of GFP, CD10, and CD19 by flow cytometry.