Table 1. Absolute Numbers of CD56 NK... | American Society of Hematology

Table 1.

Absolute Numbers of CD56⁺ NK Cells Upon Sequential Culture of Transduced CD34⁺CD38⁻ FL Cells Upon S17 and Cytokines

FL Cells Transduced With	% GFP⁺ Cells		No. of Recovered GFP⁺ Cells Per Input of 10,000 GFP⁺ Cells
FL Cells Transduced With	Before Culture	After Culture	Total	CD56⁺ Cells
IRES-GFP	12	13	43,000	10,400
Id3-IRES-GFP	8	6	30,000	12,900

FL Cells Transduced With	% GFP⁺ Cells		No. of Recovered GFP⁺ Cells Per Input of 10,000 GFP⁺ Cells
FL Cells Transduced With	Before Culture	After Culture	Total	CD56⁺ Cells
IRES-GFP	12	13	43,000	10,400
Id3-IRES-GFP	8	6	30,000	12,900

Cells were harvested after coculture of transduced FL cells on a monolayer of S17 stroma and the percentage of GFP⁺ cells was determined to get the indicated percentage of GFP⁺cells before culture. The harvested cells were further cultured in a cytokine combination consisting of Flt-3L, IL-7, and IL-15. Two weeks later, cell suspensions were analyzed by flow cytometry for GFP expression (% GFP⁺ cells after culture) and cell surface expression of the CD56 antigen. The percentage of GFP⁺ cells and therefore the absolute numbers of the input were different. To allow for a meaningful comparison, we expressed the output numbers per input number of 10,000 cells.

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