Decreased CFU-C and CFU-S Frequencies After Fas Cross-Linking on IFNγ-Induced C57BL/6 Bone Marrow Cells
| . | CFU-S/106 Cells . | CFU-C/106 Cells . |
|---|---|---|
| Control IgG | 135.4 ± 14.5 | 1,742.3 ± 176.9 |
| Anti-Fas MoAb | 153.0 ± 21.0 | 1,500.0 ± 220.7 |
| IFNγ | 126.0 ± 14.7 | 1,461.0 ± 205.3 |
| Anti-Fas MoAb + IFNγ | 39.0 ± 11.9 | 934.8 ± 164.2 |
| . | CFU-S/106 Cells . | CFU-C/106 Cells . |
|---|---|---|
| Control IgG | 135.4 ± 14.5 | 1,742.3 ± 176.9 |
| Anti-Fas MoAb | 153.0 ± 21.0 | 1,500.0 ± 220.7 |
| IFNγ | 126.0 ± 14.7 | 1,461.0 ± 205.3 |
| Anti-Fas MoAb + IFNγ | 39.0 ± 11.9 | 934.8 ± 164.2 |
Bone marrow cells (2.5 × 106/mL) were distributed into 24-well culture plates and incubated for 24 hours in the presence of control IgG (5 μg/mL), anti-Fas MoAb (5 μg/mL), mrIFNγ (100 U/mL), or anti-Fas MoAb plus mrIFNγ. CFU-S and CFU-C were then assayed as described in Materials and Methods. Data are means ± SEM from 3 and 4 separate experiments for CFU-S and CFU-C, respectively.P < .01 between anti-Fas + IFNγ and control IgG for CFU-S and P < .05 for CFU-C.