Effect of DN T Cells on Colony Formation
| Treatment . | CFU-C/106 Spleen Cells . | |
|---|---|---|
| C57BL/6 . | C57BL/6-lpr/lpr . | |
| Standard assay | 35.3 ± 8.4 | 358.5 ± 91.3 |
| +lpr/lpr LN cells | 33.2 ± 5.9 | — |
| +lpr/lpr LN supernatant | 31.9 ± 3.3 | — |
| T- and B-cell depletion | 685.3 ± 85.1 | 7,939.5 ± 256.4 |
| Treatment . | CFU-C/106 Spleen Cells . | |
|---|---|---|
| C57BL/6 . | C57BL/6-lpr/lpr . | |
| Standard assay | 35.3 ± 8.4 | 358.5 ± 91.3 |
| +lpr/lpr LN cells | 33.2 ± 5.9 | — |
| +lpr/lpr LN supernatant | 31.9 ± 3.3 | — |
| T- and B-cell depletion | 685.3 ± 85.1 | 7,939.5 ± 256.4 |
Colony-forming cells were assayed in MethoCult M3230 medium supplemented with the standard growth factor cocktail in the presence or absence of 106 lymph node cells from C57BL/6-lpr/lpr mice (50% DN T cells) or their 5-fold diluted supernatant. After magnetic depletion of B220+CD4+CD8+ cells, TCRα/β−IgM− splenocytes were electronically sorted as described in Materials and Methods. Data are means ± SEM from triplicate dishes. The experiment was performed with 3-month-old mice.
Abbreviation: LN, lymph node.