Impact of Medium Containing Serum Versus Serum-Free Medium on Mitosis, CE, Asymmetric Division, and ADI of CD34+/CD38− Cells Derived From FLV
Medium . | Mitotic Rate (%) . | CE (%) . | Asymmetric Divisions (%) . | ADI . |
---|---|---|---|---|
Serum-free | 78.7 ± 8.4 | 47.1 ± 13.8 | 31.3 ± 14.0 | 39.5 ± 16.0 |
Serum-containing | 75.4 ± 11.9 | 50.0 ± 14.0 | 36.5 ± 10.7 | 51.2 ± 10.8 |
Medium . | Mitotic Rate (%) . | CE (%) . | Asymmetric Divisions (%) . | ADI . |
---|---|---|---|---|
Serum-free | 78.7 ± 8.4 | 47.1 ± 13.8 | 31.3 ± 14.0 | 39.5 ± 16.0 |
Serum-containing | 75.4 ± 11.9 | 50.0 ± 14.0 | 36.5 ± 10.7 | 51.2 ± 10.8 |
The means ± SD are shown. Both media contained the cytokine cocktail described in text (SCF, Epo, IL-3, IL-6, GM-CSF, bFGF, and IGF-1).