Distribution of CRU Between Populations Generated in Five-Day Cultures of Fetal Liver and Cord Blood Cells Isolated According to Their Proliferation History
| Origin of CD34+ Cells Cultured . | Experiment No. . | ≤2 Divisions . | ≥3 Divisions . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Cells Injected per Mouse (×103) . | B+ and M+ . | Level of Engraftment (%) . | B+ Only . | Cells Injected per Mouse (×103) . | B+ and M+ . | Level of Engraftment (%) . | B+ Only . | ||
| Fetal liver | 4 | 5.0 | 0/5 | — | 3/5 | 900 | 3/6 | 0.1, 0.1, 2 | 6/6 |
| Fetal liver | 5 | 1.8 | 0/6 | — | 1/6 | 810 | 2/6 | 0.3, 2 | 4/6 |
| Cord blood | 4 | 6.6 | 2/6 | 0.2, 2 | 4/6 | 880 | 2/6 | 1, 1 | 6/6 |
| Cord blood | 5 | 7.8 | 1/6 | 5 | 4/6 | 1,100 | 3/6 | 1, 3, 24 | 6/6 |
| Cord blood | 6 | 6.2 | 4/6 | 0.6, 2, 2, 3 | 5/6 | 2,000 | 4/4 | 4, 7, 7, 8 | 4/4 |
| Origin of CD34+ Cells Cultured . | Experiment No. . | ≤2 Divisions . | ≥3 Divisions . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Cells Injected per Mouse (×103) . | B+ and M+ . | Level of Engraftment (%) . | B+ Only . | Cells Injected per Mouse (×103) . | B+ and M+ . | Level of Engraftment (%) . | B+ Only . | ||
| Fetal liver | 4 | 5.0 | 0/5 | — | 3/5 | 900 | 3/6 | 0.1, 0.1, 2 | 6/6 |
| Fetal liver | 5 | 1.8 | 0/6 | — | 1/6 | 810 | 2/6 | 0.3, 2 | 4/6 |
| Cord blood | 4 | 6.6 | 2/6 | 0.2, 2 | 4/6 | 880 | 2/6 | 1, 1 | 6/6 |
| Cord blood | 5 | 7.8 | 1/6 | 5 | 4/6 | 1,100 | 3/6 | 1, 3, 24 | 6/6 |
| Cord blood | 6 | 6.2 | 4/6 | 0.6, 2, 2, 3 | 5/6 | 2,000 | 4/4 | 4, 7, 7, 8 | 4/4 |
Level of engraftment (%) refers to the percent of human CD45/71+ cells detected in the marrow of mice in which both human B-lineage (CD34−CD19/20+) and myeloid (CD45/71+CD15/66b+) cells were detected. The (average) relative proportion of the total culture in each of the 2 fractions compared can be readily derived from the data shown in Fig 3. In these experiments, 83% to 90% of all the cells recovered in each experiment were used to assess the distribution of CRU between the 2 fractions being compared.