Distribution of LTC-IC Between the Undivided and Postmitotic Fractions of Cells Obtained From Five-Day Cultures of CD34+ Fetal Liver and Cord Blood Cells That Had Not Divided During This Period (Undivided Cells) and That Had (Postmitotic)
Origin of CD34+ Cells Cultured . | Experiment No. . | LTC-IC (per 103 cells) . | % of Total LTC-IC . | ||
---|---|---|---|---|---|
Undivided . | Postmitotic . | Undivided . | Postmitotic . | ||
Fetal liver | 1 | 190 | 0.4 | 11 | 89 |
Fetal liver | 2 | <1.5 | 14 | <0.1 | >99.9 |
Fetal liver | 3 | 0.2 | 14 | <0.1 | >99.9 |
Cord blood | 1 | <4 | 1.0 | <0.6 | >99 |
Cord blood | 2 | 1.9 | 34 | 0.01 | 99 |
Cord blood | 3 | 82 | 1.7 | 1.5 | 98 |
Origin of CD34+ Cells Cultured . | Experiment No. . | LTC-IC (per 103 cells) . | % of Total LTC-IC . | ||
---|---|---|---|---|---|
Undivided . | Postmitotic . | Undivided . | Postmitotic . | ||
Fetal liver | 1 | 190 | 0.4 | 11 | 89 |
Fetal liver | 2 | <1.5 | 14 | <0.1 | >99.9 |
Fetal liver | 3 | 0.2 | 14 | <0.1 | >99.9 |
Cord blood | 1 | <4 | 1.0 | <0.6 | >99 |
Cord blood | 2 | 1.9 | 34 | 0.01 | 99 |
Cord blood | 3 | 82 | 1.7 | 1.5 | 98 |
Postmitotic refers to all cells that had undergone ≥1 division as indicated by their relative CFSE fluorescence.