Changes in CFC and LTC-IC Numbers After Culturing CD34+ Fetal Liver and Cord Blood Cells for Five Days in the Presence of IL-3, IL-6, SCF, G-SCF, and FL
| Origin of CD34+ Cells Cultured . | Experiment No. . | Progenitor Input (per 103 cells) . | Progenitor Yield (per initial 103 cells) . | Fold Expansion . | |||
|---|---|---|---|---|---|---|---|
| CFC . | LTC-IC . | CFC . | LTC-IC . | CFC . | LTC-IC . | ||
| Fetal liver | 1 | 300 | 11 | 12,000 | 46 | 40 | 4.2 |
| Fetal liver | 2 | 210 | 24 | 6,900 | 260 | 33 | 11 |
| Fetal liver | 3 | 100 | 28 | 3,400 | 160 | 34 | 5.7 |
| Fetal liver | 4 | 180 | 16 | 5,800 | 41 | 32 | 2.8 |
| Fetal liver | 5 | 150 | 23 | 5,400 | 118 | 36 | 5.1 |
| Mean ± SEM | 188 ± 33 | 20 ± 3 | 6,700 ± 1,440 | 125 ± 40 | 35 ± 1 | 6 ± 1 | |
| Cord blood | 1 | ND | 170 | ND | 240 | — | 1.4 |
| Cord blood | 2 | 200 | 11 | 7,600 | 82 | 38 | 7.4 |
| Cord blood | 3 | 300 | 2 | 9,200 | 140 | 31 | 70 |
| Cord blood | 4 | 260 | 12 | 6,700 | 8 | 26 | 0.7 |
| Cord blood | 5 | 240 | 0.4 | 8,400 | 15 | 35 | 40 |
| Cord blood | 6 | 230 | 3 | 10,000 | 64 | 44 | 21 |
| Mean ± SEM | 246 ± 17 | 33 ± 27 | 8,380 ± 580 | 91 ± 36 | 35 ± 3 | 23 ± 11 | |
| Origin of CD34+ Cells Cultured . | Experiment No. . | Progenitor Input (per 103 cells) . | Progenitor Yield (per initial 103 cells) . | Fold Expansion . | |||
|---|---|---|---|---|---|---|---|
| CFC . | LTC-IC . | CFC . | LTC-IC . | CFC . | LTC-IC . | ||
| Fetal liver | 1 | 300 | 11 | 12,000 | 46 | 40 | 4.2 |
| Fetal liver | 2 | 210 | 24 | 6,900 | 260 | 33 | 11 |
| Fetal liver | 3 | 100 | 28 | 3,400 | 160 | 34 | 5.7 |
| Fetal liver | 4 | 180 | 16 | 5,800 | 41 | 32 | 2.8 |
| Fetal liver | 5 | 150 | 23 | 5,400 | 118 | 36 | 5.1 |
| Mean ± SEM | 188 ± 33 | 20 ± 3 | 6,700 ± 1,440 | 125 ± 40 | 35 ± 1 | 6 ± 1 | |
| Cord blood | 1 | ND | 170 | ND | 240 | — | 1.4 |
| Cord blood | 2 | 200 | 11 | 7,600 | 82 | 38 | 7.4 |
| Cord blood | 3 | 300 | 2 | 9,200 | 140 | 31 | 70 |
| Cord blood | 4 | 260 | 12 | 6,700 | 8 | 26 | 0.7 |
| Cord blood | 5 | 240 | 0.4 | 8,400 | 15 | 35 | 40 |
| Cord blood | 6 | 230 | 3 | 10,000 | 64 | 44 | 21 |
| Mean ± SEM | 246 ± 17 | 33 ± 27 | 8,380 ± 580 | 91 ± 36 | 35 ± 3 | 23 ± 11 | |
Fold expansion values were calculated by dividing the progenitor yield (at the end of a 5-day culture in serum-free medium plus FL, SF, IL-3, IL-6, and G-CSF) by the initial progenitor content of the corresponding culture.