Table 2. Colony Numbers of Sorted BM... | American Society of Hematology

Table 2.

Colony Numbers of Sorted BM CD34⁺97A6⁺ and CD34⁺97A6⁻ Cells

Fraction	BFU-E	CFU-GM	CFU-Baso^*
CD34⁺	33.4 ± 7.4	47.5 ± 8.4	nd
CD34⁺97A6⁺	0.5 ± 0.5	12.5 ± 3.5	85 ± 5%
CD34⁺97A6⁻	46.2 ± 6.8	37.3 ± 5.2	nd

Fraction	BFU-E	CFU-GM	CFU-Baso^*
CD34⁺	33.4 ± 7.4	47.5 ± 8.4	nd
CD34⁺97A6⁺	0.5 ± 0.5	12.5 ± 3.5	85 ± 5%
CD34⁺97A6⁻	46.2 ± 6.8	37.3 ± 5.2	nd

Colony-forming capacity of MACS-selected CD34⁺ BM cells stained with anti-CD34-FITC and 97A6-PE and FACS-sorted into CD34⁺97A6⁺ and CD34⁺97A6⁻ fractions. Fractionated cells were plated in semisolid methylcellulose cultures as described in Materials and Methods. Cultures were scored for colony growth after 16 days of incubation at 37°C and 5% CO₂. Results represent the mean colony numbers ± standard deviation (SD) based on 1 × 10³ plated cells from 2 independent experiments with duplicate determinations. For determination of basophil colonies in the CD34⁺97A6⁺ fraction, single colonies were transferred with a Pasteur pipette on glass slides, air-dried, and stained with May-Grünwald-Giemsa for morphological analysis.

Abbreviations: BFU-E, burst-forming units-erythrocyte; CFU-GM, colony-forming units-granulocyte/macrophage; CFU-Baso, colony-forming units-basophils; nd, not determined.

CFU-Baso or mixed colonies in which basophils were detected.

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