Table 1. Determination of Mast Cell and... | American Society of Hematology

Table 1.

Determination of Mast Cell and Tryptase Content in CD34⁺ BM Cells Stimulated With 100 ng/mL SCF in Liquid Culture for 35 Days

	CD34⁺	CD34⁺97A6⁺	CD34⁺97A6⁻
Cell count	20 × 10⁴	3.5 × 10⁴	8.5 × 10⁴
% mast cells	30%	36%	32%
Total number of mast cells	6 × 10⁴	1.26 × 10⁴	2.72 × 10⁴
Tryptase/10⁴ mast cells	15.16 ng	11.19 ng	14.19 ng

	CD34⁺	CD34⁺97A6⁺	CD34⁺97A6⁻
Cell count	20 × 10⁴	3.5 × 10⁴	8.5 × 10⁴
% mast cells	30%	36%	32%
Total number of mast cells	6 × 10⁴	1.26 × 10⁴	2.72 × 10⁴
Tryptase/10⁴ mast cells	15.16 ng	11.19 ng	14.19 ng

One thousand cells of each BM fraction (CD34⁺, CD34⁺97A6⁺, and CD34⁺97A6⁻) were cultured in the presence of 100 ng/mL SCF and 10 ng/mL for 35 days. After days 14 and 28 of culture, the medium was replaced with serum-free medium containing 100 ng/mL SCF. After culture, cells were counted and mast cells were identified by morphology. The tryptase content was determined as described in Materials and Methods.

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