Effect of Transduced c-kit on c-kitExpression on Subsets of CD34+++ CB Cells Analyzed by Flow Cytometry
| Subsets of CD34+++ Cells . | Percent c-kit Expressing Cells . | ||
|---|---|---|---|
| M5g Neo . | M5g hkit Neo . | Percent Change . | |
| Kit++ | 45.2 ± 14 | 53.1 ± 15 | +17 |
| Kit+ | 21.1 ± 4 | 46.0 ± 17 | +118 |
| KitLo/− | 7.2 ± 3 | 14.5 ± 4 | +101 |
| Subsets of CD34+++ Cells . | Percent c-kit Expressing Cells . | ||
|---|---|---|---|
| M5g Neo . | M5g hkit Neo . | Percent Change . | |
| Kit++ | 45.2 ± 14 | 53.1 ± 15 | +17 |
| Kit+ | 21.1 ± 4 | 46.0 ± 17 | +118 |
| KitLo/− | 7.2 ± 3 | 14.5 ± 4 | +101 |
Sorted CD34+++ kit++, kit+, and kitLo/− cells were prestimulated with GM-CSF (200 U), IL-3 (200 U), Epo (1 U), and IL-6 (10 ng) per mL without SLF and transduced with c-kit (M5g hkit Neo) or mock (M5g neo) viruses as described in Materials and Methods. The cells were washed and incubated with the same cytokine combination for 48 hours. Cells were washed and stained with CD117/PE or isotope control and analyzed by flow cytometer. Results are expressed as mean percent of c-kitexpressing cells ± SEM from an average of 4 separate experiments.