Relative hCD4 Level Per Cell in Different Cell Types
| Cell Type* . | vav-hCD4Transgene Construct† . | |
|---|---|---|
| HS21/45 . | HS321/45 . | |
| Adult | ||
| T lymphoid | 100‡ | 100 |
| B lymphoid | 72 ± 17 | 120 ± 20 |
| Myeloid | 31 ± 7 | 22 ± 4 |
| Erythroid | 7 ± 4 | 8 ± 2 |
| Fetal | ||
| Nonerythroid | 52 | 160 |
| Definitive erythroid | 2 | 8 |
| Primitive erythroid | <1 | <3 |
| Cell Type* . | vav-hCD4Transgene Construct† . | |
|---|---|---|
| HS21/45 . | HS321/45 . | |
| Adult | ||
| T lymphoid | 100‡ | 100 |
| B lymphoid | 72 ± 17 | 120 ± 20 |
| Myeloid | 31 ± 7 | 22 ± 4 |
| Erythroid | 7 ± 4 | 8 ± 2 |
| Fetal | ||
| Nonerythroid | 52 | 160 |
| Definitive erythroid | 2 | 8 |
| Primitive erythroid | <1 | <3 |
Cell types were distinguished, as in Fig 3, by FACS analysis for surface markers: T lymphoid (Thy-1+), B lymphoid (B220+), myeloid (Mac-1+), and erythroid (Ter119+).
The data presented, derived from multiple FACS analyses like those in Figs 3 and 5, are the means (±SD) of hCD4-associated fluorescence (in relative fluorescence intensity units) above the background in nontransgenic mice, normalized to the level in T cells (set at 100) within each transgenic strain. Data for adult bone marrow, spleen, and thymus were from 5 HS21/45 and 3 HS321/45 strains; fetal liver (E14.5) and blood (E10.5) from 1 HS21/45 and 1 HS321/45 strain.
The actual mean fluorescence values were 300 ± 250 for the HS21/45 strains and 190 ± 50 for the HS321/45 strains.