Primer Sequences, Chromosomal Location, and Size of Expected PCR Products
| Primer Pair . | Sequence . | Locus . | Size . |
|---|---|---|---|
| D2S123-for | 5′-CCT TTC TGA CTT GGA TAC CAT CTA TCT ATC TA-3′ | 2 | 157-187 |
| D2S123-rev | 5′-CAG GAT GCC TGC CTT TAA CAG TG-3′ | ||
| D9S126-for | 5′-ATT GAA ACT CTG CTG AAT TTT CTG-3′ | 9p21 | 238-248 |
| D9S126-rev | 5′-CAA CTC CTC TTG GGA ACT GC-3′ | ||
| D10S197-for | 5′-AGC TGA GAT CGC ACC ACT GCA CTT CAG-3′ | 10 | 161-173 |
| D10S197-rev | 5′-AGG GTA GCC TTT CCT ATC CTC CCA TTC-3′ | ||
| VW-for | 5′-CCC TAG TGG ATG ATA AGA ATA ATC-3′ | 12p12 | 138-162 |
| VW-rev | 5′-GGA CAG ATG ATA AAT ACA TAG GAT GGA ATG G-3′ | ||
| D15S212-for | 5′-CAG ATT TCA GAT GTG CCT AGT CCA C-3′ | 15 | 203-217 |
| D15S212-rev | 5′-CCT TAG CCA GTG AGT GCA TGT G-3′ | ||
| D15S161-for | 5′-TCTGTGATTTTGCCATTATGAG-3′ | 15 | 277-291 |
| D15S161-rev | 5′-TAAACTGGAATTTTTGACTATGAGC-3′ | ||
| D18S61-for | 5′-ATT TCT AAG AGG ACT CCC AAA CT-3′ | 18 | 157-183 |
| D18S61-rev | 5′-ATA TTT TGA AAC TCA GGA GCA T-3′ | ||
| D18S65-for | 5′-AAT AAG TTT GGA AGC AGG TGG AG-3′ | 18 | 168-178 |
| D18S65-rev | 5′-AGA GGA GAG GCT GGT CTT ACT AT-3′ | ||
| AR-for | 5′-TCC GCG AAG TGA TCC AGA AC-3′ | Xq11-12 | 213-225 |
| AR-rev | 5′-CTT GGG GAG AAC CAT CCT CA-3′ | ||
| DxS1217-for | 5′-TCC AGT AAG TTT GGT CTA TAT GAC G-3′ | X | 231-243 |
| DxS1217-rev | 5′-ATG AAG TAT CGT ATC TGA ATC CCG-3′ |
| Primer Pair . | Sequence . | Locus . | Size . |
|---|---|---|---|
| D2S123-for | 5′-CCT TTC TGA CTT GGA TAC CAT CTA TCT ATC TA-3′ | 2 | 157-187 |
| D2S123-rev | 5′-CAG GAT GCC TGC CTT TAA CAG TG-3′ | ||
| D9S126-for | 5′-ATT GAA ACT CTG CTG AAT TTT CTG-3′ | 9p21 | 238-248 |
| D9S126-rev | 5′-CAA CTC CTC TTG GGA ACT GC-3′ | ||
| D10S197-for | 5′-AGC TGA GAT CGC ACC ACT GCA CTT CAG-3′ | 10 | 161-173 |
| D10S197-rev | 5′-AGG GTA GCC TTT CCT ATC CTC CCA TTC-3′ | ||
| VW-for | 5′-CCC TAG TGG ATG ATA AGA ATA ATC-3′ | 12p12 | 138-162 |
| VW-rev | 5′-GGA CAG ATG ATA AAT ACA TAG GAT GGA ATG G-3′ | ||
| D15S212-for | 5′-CAG ATT TCA GAT GTG CCT AGT CCA C-3′ | 15 | 203-217 |
| D15S212-rev | 5′-CCT TAG CCA GTG AGT GCA TGT G-3′ | ||
| D15S161-for | 5′-TCTGTGATTTTGCCATTATGAG-3′ | 15 | 277-291 |
| D15S161-rev | 5′-TAAACTGGAATTTTTGACTATGAGC-3′ | ||
| D18S61-for | 5′-ATT TCT AAG AGG ACT CCC AAA CT-3′ | 18 | 157-183 |
| D18S61-rev | 5′-ATA TTT TGA AAC TCA GGA GCA T-3′ | ||
| D18S65-for | 5′-AAT AAG TTT GGA AGC AGG TGG AG-3′ | 18 | 168-178 |
| D18S65-rev | 5′-AGA GGA GAG GCT GGT CTT ACT AT-3′ | ||
| AR-for | 5′-TCC GCG AAG TGA TCC AGA AC-3′ | Xq11-12 | 213-225 |
| AR-rev | 5′-CTT GGG GAG AAC CAT CCT CA-3′ | ||
| DxS1217-for | 5′-TCC AGT AAG TTT GGT CTA TAT GAC G-3′ | X | 231-243 |
| DxS1217-rev | 5′-ATG AAG TAT CGT ATC TGA ATC CCG-3′ |