The Effect of Selenite Pretreatment on As2O3-Induced Apoptosis and GPx Activity in NB4 Cells
| Cells . | As2O3 (μmol/L) . | Apoptosis (%) . | GPx (mU/min/mg protein) . |
|---|---|---|---|
| NB4 | 0 | 2.1 ± 0.4 | 24.5 ± 5.1 |
| 0.5 | 12.4 ± 3.8 | 13.5 ± 2.8 | |
| 1 | 57.8 ± 7.2 | 8.9 ± 4.2 | |
| NB4-sele | 0 | 2.3 ± 0.8 | 166.4 ± 14.9 |
| 0.5 | 5.8 ± 3.1 | 135.2 ± 10.8 | |
| 1 | 7.1 ± 2.7 | 117.4 ± 20.2 |
| Cells . | As2O3 (μmol/L) . | Apoptosis (%) . | GPx (mU/min/mg protein) . |
|---|---|---|---|
| NB4 | 0 | 2.1 ± 0.4 | 24.5 ± 5.1 |
| 0.5 | 12.4 ± 3.8 | 13.5 ± 2.8 | |
| 1 | 57.8 ± 7.2 | 8.9 ± 4.2 | |
| NB4-sele | 0 | 2.3 ± 0.8 | 166.4 ± 14.9 |
| 0.5 | 5.8 ± 3.1 | 135.2 ± 10.8 | |
| 1 | 7.1 ± 2.7 | 117.4 ± 20.2 |
NB4-sele cells were pretreated with 0.1 μmol/L sodium selenite for 9 days and were then treated with As2O3 for 3 days. Apoptosis (determined by fluorescence microscopy) and GPx activity were assayed and determined after 3 days of treatment with or without As2O3. Each value is the mean ± SD of triplicate determinations.