Ex Vivo Culture and Transduction of Rhesus Lymphocytes
| Rhesus Monkeys . | AAV (MOI*) . | Cell Numbers (in millions) . | Expansion (fold increase over starting no.) . | In Vitro Transduction Efficiency (mean copy no. per cell) . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| AAV . | RV . | |||||||||
| Day 0 (harvest) . | Day 3 (split) . | Day 10 (infusion) . | AAV . | RV . | Total . | PCR . | PCR . | Southern . | ||
| 94E068 | AAVPLO (2,000) | 200 | 120 | 740 | 5.4 | 2.0 | 3.7 | 0.015 | .13 | .14 |
| RQ1307 | AAVPLO (20,000) | 97 | 46 | 890 | 11.3 | 7.0 | 9.2 | .17 | .19 | .22 |
| RQ1303 | AAVPLIII (20,000) | 117 | 58 | 960 | 8.0 | 8.4 | 8.2 | .80 | .14 | .17 |
| RQ854 | AAVLPO (200,000) | 299 | 96 | 206 | 0.41 | 0.97 | 0.7 | 3.0 | .15 | .16 |
| Mean | NA | 178 | 80 | 699 | 4.5 | 3.3 | 3.9 | NA | .15 | .17 |
| Rhesus Monkeys . | AAV (MOI*) . | Cell Numbers (in millions) . | Expansion (fold increase over starting no.) . | In Vitro Transduction Efficiency (mean copy no. per cell) . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| AAV . | RV . | |||||||||
| Day 0 (harvest) . | Day 3 (split) . | Day 10 (infusion) . | AAV . | RV . | Total . | PCR . | PCR . | Southern . | ||
| 94E068 | AAVPLO (2,000) | 200 | 120 | 740 | 5.4 | 2.0 | 3.7 | 0.015 | .13 | .14 |
| RQ1307 | AAVPLO (20,000) | 97 | 46 | 890 | 11.3 | 7.0 | 9.2 | .17 | .19 | .22 |
| RQ1303 | AAVPLIII (20,000) | 117 | 58 | 960 | 8.0 | 8.4 | 8.2 | .80 | .14 | .17 |
| RQ854 | AAVLPO (200,000) | 299 | 96 | 206 | 0.41 | 0.97 | 0.7 | 3.0 | .15 | .16 |
| Mean | NA | 178 | 80 | 699 | 4.5 | 3.3 | 3.9 | NA | .15 | .17 |
Abbreviations: RV, retrovirus; NA, not available.
MOI indicates viral genomic copy number per lymphocyte.