Analysis of EphA3 Expression in Human Hematopoietic Cell Lines
| Hematopoietic Cell Line . | Cell Lineage . | FCA . | QMA (no./cell) . | Real-Time PCR* . |
|---|---|---|---|---|
| HPB-ALL | T cell (thymic) | − | <240 | |
| HSB-2 | T cell (thymic) | ± | 1,070† | 0.02 |
| JM | T cell | + | 6,800 | 0.11 |
| Jurkat | T cell | + | NT | |
| MOLT4 | T cell | + | 16,900 | |
| LILA-1 | Pre-B | − | <240 | |
| LK63 | Pre-B | ++ | 78,000 | 1.0 |
| Nalm 1 | Pre-B | − | NT | |
| Raji | B cell | − | <240 | <0.001 |
| HL60 | Myelo-monocytic | − | <240 | |
| RC2a | Myelo-monocytic | − | <240 | |
| U937 | Monocytic | − | <240 | |
| HEL | Megakarocytic | ± | 8,300 | |
| K562 | Erythroid | − | <240 |
| Hematopoietic Cell Line . | Cell Lineage . | FCA . | QMA (no./cell) . | Real-Time PCR* . |
|---|---|---|---|---|
| HPB-ALL | T cell (thymic) | − | <240 | |
| HSB-2 | T cell (thymic) | ± | 1,070† | 0.02 |
| JM | T cell | + | 6,800 | 0.11 |
| Jurkat | T cell | + | NT | |
| MOLT4 | T cell | + | 16,900 | |
| LILA-1 | Pre-B | − | <240 | |
| LK63 | Pre-B | ++ | 78,000 | 1.0 |
| Nalm 1 | Pre-B | − | NT | |
| Raji | B cell | − | <240 | <0.001 |
| HL60 | Myelo-monocytic | − | <240 | |
| RC2a | Myelo-monocytic | − | <240 | |
| U937 | Monocytic | − | <240 | |
| HEL | Megakarocytic | ± | 8,300 | |
| K562 | Erythroid | − | <240 |
EphA3 expression and receptor number (no.) per cell was measured using FCA and QMA. EphA3 expression by FCA is shown as being either strongly positive (++), positive (+), weakly positive (±), or negative (−).
Real-time PCR was used to estimate the relative amount of EphA3 mRNA in selected cell lines; results are normalized to the content in LK63 cells.
Receptor number for HSB-2 taken from Wicks et al, 1992.14