Production of CFC From Lin−CD34−CD38− Adult Human Marrow or Mobilized Peripheral Blood Cells in 10-Day Cultures Containing Various Cytokines
| Sample . | Phenotype . | No. of Sorts . | Cytokine Combination . | |||
|---|---|---|---|---|---|---|
| F + S + T + 3 + H6 . | F + S + 3 . | F + T + H6 . | F + S + T + H6 . | |||
| mPB1 | CD34−CD38− | 1 | 50 | 0 | 0 | ND |
| CD34+CD38− | 1 | 6,900 | 1,100 | 650 | ND | |
| BM2 | CD34−CD38− | 1 | 1,300 | 0 | 0 | ND |
| CD34+CD38− | 1 | 4,600 | 2,100 | 860 | ND | |
| BM1 | CD34−CD38− | 1 | 40 | ND | ND | ND |
| BM3 | CD34−CD38− | 1 | 2,100 | 0 | 0 | 650 |
| BM8 | CD34−CD38− | 1 | 140 | ND | ND | ND |
| BM4 | CD34−CD38− | 2 | 120 | ND | ND | ND |
| BM5 | CD34−CD38− | 2 | 440 | ND | ND | ND |
| BM6 | CD34−CD38− | 2 | 920 | ND | ND | ND |
| BM7 | CD34−CD38− | 2 | 500 | ND | ND | ND |
| Mean ± SEM | CD34−CD38− | 1 | 730 ± 420 | |||
| CD34−CD38− | 2 | 500 ± 160 | ||||
| Sample . | Phenotype . | No. of Sorts . | Cytokine Combination . | |||
|---|---|---|---|---|---|---|
| F + S + T + 3 + H6 . | F + S + 3 . | F + T + H6 . | F + S + T + H6 . | |||
| mPB1 | CD34−CD38− | 1 | 50 | 0 | 0 | ND |
| CD34+CD38− | 1 | 6,900 | 1,100 | 650 | ND | |
| BM2 | CD34−CD38− | 1 | 1,300 | 0 | 0 | ND |
| CD34+CD38− | 1 | 4,600 | 2,100 | 860 | ND | |
| BM1 | CD34−CD38− | 1 | 40 | ND | ND | ND |
| BM3 | CD34−CD38− | 1 | 2,100 | 0 | 0 | 650 |
| BM8 | CD34−CD38− | 1 | 140 | ND | ND | ND |
| BM4 | CD34−CD38− | 2 | 120 | ND | ND | ND |
| BM5 | CD34−CD38− | 2 | 440 | ND | ND | ND |
| BM6 | CD34−CD38− | 2 | 920 | ND | ND | ND |
| BM7 | CD34−CD38− | 2 | 500 | ND | ND | ND |
| Mean ± SEM | CD34−CD38− | 1 | 730 ± 420 | |||
| CD34−CD38− | 2 | 500 ± 160 | ||||
Cells (sorted once or twice, as indicated) were cultured (usually 500 cells per 100 μL × 2) for 10 days in serum-free medium with various cytokines (see text) and then CFC assays performed, usually by plating 10% of the harvested cells per 1 mL CFC assay. The data shown have been rounded off to 2 significant figures. More precise data on the distribution of the different types of CFC detected are shown in Table 4.
Abbreviations: ND, not done; mPB, mobilized peripheral blood; BM, bone marrow (number indicates sample number); F, FL; S, SF; 3, IL-3; H6, H–IL-6; T, TPO.