Proportions of Light Density and Lin−Marrow and Mobilized Blood Cells That Are Lin−CD34+, Lin−CD34+CD38−, or Lin−CD34−CD38−
| Tissue . | Fraction . | % Lin−CD34+ Cells . | % Lin− CD34+CD38− Cells . | % Lin− CD34−CD38− Cells . |
|---|---|---|---|---|
| Adult marrow (n = 5) | Low density | 2.6 ± 0.6 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| Lin− | 28 ± 5 | 2.4 ± 0.8 | 3.6 ± 1.3 | |
| Mobilized blood (n = 8) | Low density | 1.9 ± 0.8 | 0.12 ± 0.04 | 0.3 ± 0.1 |
| Lin− | 41 ± 8 | 3.2 ± 1.3 | 6 ± 2 |
| Tissue . | Fraction . | % Lin−CD34+ Cells . | % Lin− CD34+CD38− Cells . | % Lin− CD34−CD38− Cells . |
|---|---|---|---|---|
| Adult marrow (n = 5) | Low density | 2.6 ± 0.6 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| Lin− | 28 ± 5 | 2.4 ± 0.8 | 3.6 ± 1.3 | |
| Mobilized blood (n = 8) | Low density | 1.9 ± 0.8 | 0.12 ± 0.04 | 0.3 ± 0.1 |
| Lin− | 41 ± 8 | 3.2 ± 1.3 | 6 ± 2 |
The frequency of lin−CD34+, lin−CD34+CD38−, and lin−CD34−CD38− cells in the lin− population was determined by FACS analysis. These values were then used to calculate the frequency of these 2 cell types in the original low-density fraction, assuming equivalent nonspecific losses of both cell types during the StemSep separation procedure used to isolate the lin− preparation. Values shown are the mean ± SEM.