Hybrid BCR/ABL Molecules in Ph+ Bone Marrow Mononuclear Cells of IFN- Responder and Nonresponder CML Patients Enrolled in the Study
| Case . | Hybrid BCR/ABL mRNA Junction . | At Diagnosis . | At Hematologic Remission . | Hematologic Response to IFN . | ||
|---|---|---|---|---|---|---|
| % of Ph+ Cells . | Molecules of BCR/ABL mRNA* . | % of Ph+ Cells . | Molecules of BCR/ABL mRNA* . | |||
| 1 | b3/a2 | 90 | 425 × 103 | 80 | 228 × 103 | Complete |
| 2 | b3/a2 | 100 | 151 × 103 | 100 | 12 × 103 | Complete |
| 3 | b2/a2 | 100 | 179 × 103 | 100 | 18 × 103 | Complete |
| 4 | b2/a2 | 100 | 57 × 103 | 100 | 17 × 103 | Complete |
| 5 | b2/a2 | 100 | 74 × 103 | 100 | 24 × 103 | Complete |
| 6 | b2/a2 | 100 | 135 × 103 | 100 | 65 × 103 | Complete |
| 7 | b3/a2 | 100 | 59 × 103 | 100 | 15 × 103 | Complete |
| 8 | b2/a2 | 100 | 438 × 103 | 95 | 47 × 103 | Complete |
| 9 | b3/a2 | 100 | 122 × 103 | 100 | 57 × 103 | Complete |
| 10 | b3/a2 | 85 | 157 × 103 | 75 | 60 × 103 | Complete |
| 11 | b3/a2 | 100 | 58 × 103 | 100 | 12 × 103 | Complete |
| 12 | b2/a2 | 100 | 145 × 103 | 100 | 72 × 103 | Complete |
| 13 | b3/a2 | 100 | 198 × 103 | 100 | 42 × 103 | Complete |
| 14 | b2/a2 | 100 | 122 × 103 | 100 | 37 × 103 | Complete |
| x ± SD | 166 × 103 ± 117 × 103 | 50 × 103 ± 53 × 103 | ||||
| 15 | b2/a2 | 100 | 94 × 103 | 100 | 1,093 × 103 | No response |
| 16 | b3/a2 | 100 | 357 × 103 | 100 | 984 × 103 | No response |
| 17 | b2/a2 | 100 | 112 × 103 | 100 | 205 × 103 | No response |
| 18 | b3/a2 | 100 | 110 × 103 | 100 | 470 × 103 | No response |
| 19 | b2/a2 | 100 | 81 × 103 | 100 | 164 × 103 | No response |
| 20 | b3/a2 | 100 | 144 × 103 | 100 | 178 × 103 | No response |
| x ± SD | 150 × 103 ± 95 × 103 | 516 × 103 ± 385 × 103 | ||||
| Case . | Hybrid BCR/ABL mRNA Junction . | At Diagnosis . | At Hematologic Remission . | Hematologic Response to IFN . | ||
|---|---|---|---|---|---|---|
| % of Ph+ Cells . | Molecules of BCR/ABL mRNA* . | % of Ph+ Cells . | Molecules of BCR/ABL mRNA* . | |||
| 1 | b3/a2 | 90 | 425 × 103 | 80 | 228 × 103 | Complete |
| 2 | b3/a2 | 100 | 151 × 103 | 100 | 12 × 103 | Complete |
| 3 | b2/a2 | 100 | 179 × 103 | 100 | 18 × 103 | Complete |
| 4 | b2/a2 | 100 | 57 × 103 | 100 | 17 × 103 | Complete |
| 5 | b2/a2 | 100 | 74 × 103 | 100 | 24 × 103 | Complete |
| 6 | b2/a2 | 100 | 135 × 103 | 100 | 65 × 103 | Complete |
| 7 | b3/a2 | 100 | 59 × 103 | 100 | 15 × 103 | Complete |
| 8 | b2/a2 | 100 | 438 × 103 | 95 | 47 × 103 | Complete |
| 9 | b3/a2 | 100 | 122 × 103 | 100 | 57 × 103 | Complete |
| 10 | b3/a2 | 85 | 157 × 103 | 75 | 60 × 103 | Complete |
| 11 | b3/a2 | 100 | 58 × 103 | 100 | 12 × 103 | Complete |
| 12 | b2/a2 | 100 | 145 × 103 | 100 | 72 × 103 | Complete |
| 13 | b3/a2 | 100 | 198 × 103 | 100 | 42 × 103 | Complete |
| 14 | b2/a2 | 100 | 122 × 103 | 100 | 37 × 103 | Complete |
| x ± SD | 166 × 103 ± 117 × 103 | 50 × 103 ± 53 × 103 | ||||
| 15 | b2/a2 | 100 | 94 × 103 | 100 | 1,093 × 103 | No response |
| 16 | b3/a2 | 100 | 357 × 103 | 100 | 984 × 103 | No response |
| 17 | b2/a2 | 100 | 112 × 103 | 100 | 205 × 103 | No response |
| 18 | b3/a2 | 100 | 110 × 103 | 100 | 470 × 103 | No response |
| 19 | b2/a2 | 100 | 81 × 103 | 100 | 164 × 103 | No response |
| 20 | b3/a2 | 100 | 144 × 103 | 100 | 178 × 103 | No response |
| x ± SD | 150 × 103 ± 95 × 103 | 516 × 103 ± 385 × 103 | ||||
The number of BCR/ABL molecules are expressed per microgram of total RNA and are adjusted to the relative proportion of Ph+ cells, as evaluated by Southern analysis.