A Proportion of CD34+ Normal Bone Marrow (NBM) and CML Cells Can Remain Viable and Quiescent for at Least 4 Days When Cultured in the Presence or Absence of Growth Factors
| Growth Factors Added . | Cell Type Assessed . | % of Input Cells, or CFC, or LTC-IC Remaining Quiescent for 4 Days . | ||
|---|---|---|---|---|
| NBM . | CML . | (% Ph+) . | ||
| None | Total cells | 93 ± 1.4 | 38 ± 15 | (100 ± 0, n = 3) |
| CFC | 100 ± 0 | 7 ± 6 | (100, n = 2) | |
| LTC-IC | 100 ± 0 | ND | ||
| TPO | Total cells | 42 ± 17 | 15 ± 6 | (84 ± 32, n = 3) |
| CFC | 43 ± 22 | 9 ± 11 | (100 ± 0, n = 6) | |
| LTC-IC | 97 (n = 1) | ND | ||
| FL, SF, IL-3, IL-6, G-CSF | Total cells CFC LTC-IC | 18 ± 19 1 ± 1 92 (n = 1) | 2.0 ± 0.5 1 ± 1 ND | (100, n = 1) |
| Growth Factors Added . | Cell Type Assessed . | % of Input Cells, or CFC, or LTC-IC Remaining Quiescent for 4 Days . | ||
|---|---|---|---|---|
| NBM . | CML . | (% Ph+) . | ||
| None | Total cells | 93 ± 1.4 | 38 ± 15 | (100 ± 0, n = 3) |
| CFC | 100 ± 0 | 7 ± 6 | (100, n = 2) | |
| LTC-IC | 100 ± 0 | ND | ||
| TPO | Total cells | 42 ± 17 | 15 ± 6 | (84 ± 32, n = 3) |
| CFC | 43 ± 22 | 9 ± 11 | (100 ± 0, n = 6) | |
| LTC-IC | 97 (n = 1) | ND | ||
| FL, SF, IL-3, IL-6, G-CSF | Total cells CFC LTC-IC | 18 ± 19 1 ± 1 92 (n = 1) | 2.0 ± 0.5 1 ± 1 ND | (100, n = 1) |
Three normal marrow samples (NBM) and different numbers of CML samples (as shown) were assessed (no growth factors, n = 3; +TPO, n = 8; +5 growth factors, n = 2) except as shown. Values shown are the mean ± SEM. Genotyping was not performed (or not successful) on all CML samples assayed.
Abbreviation: ND, no data.