Concentrations of TPO in the Culture Supernatants of Carcinoma Cells
| Cell Line . | Concentration of TPO (fmol/mL) . | |
|---|---|---|
| IL-1α (−) . | IL-1α (+)4-150 . | |
| T24 | 0.34 ± 0.05 | 0.28 ± 0.08 |
| HepG2 | 5.86 ± 0.44 | 5.38 ± 0.54 |
| UT-M1 | 0.44 ± 0.14 | 0.34 ± 0.06 |
| RERF-LC-MS | 0.25 ± 0.06 | 0.29 ± 0.07 |
| 86-2 | 0.24 ± 0.05 | 0.33 ± 0.08 |
| LK-79 | 0.29 ± 0.05 | 0.28 ± 0.06 |
| LU65 | 0.41 ± 0.12 | 0.53 ± 0.14 |
| VMRC-LCD | 0.78 ± 0.18 | 0.89 ± 0.14 |
| SK-HEP-1 | <0.20 | ND |
| Lc-1sq | <0.20 | ND |
| FCS | <0.20 | ND |
| RPMI/10% FCS | <0.20 | ND |
| Cell Line . | Concentration of TPO (fmol/mL) . | |
|---|---|---|
| IL-1α (−) . | IL-1α (+)4-150 . | |
| T24 | 0.34 ± 0.05 | 0.28 ± 0.08 |
| HepG2 | 5.86 ± 0.44 | 5.38 ± 0.54 |
| UT-M1 | 0.44 ± 0.14 | 0.34 ± 0.06 |
| RERF-LC-MS | 0.25 ± 0.06 | 0.29 ± 0.07 |
| 86-2 | 0.24 ± 0.05 | 0.33 ± 0.08 |
| LK-79 | 0.29 ± 0.05 | 0.28 ± 0.06 |
| LU65 | 0.41 ± 0.12 | 0.53 ± 0.14 |
| VMRC-LCD | 0.78 ± 0.18 | 0.89 ± 0.14 |
| SK-HEP-1 | <0.20 | ND |
| Lc-1sq | <0.20 | ND |
| FCS | <0.20 | ND |
| RPMI/10% FCS | <0.20 | ND |
Cells from each cell line were cultured at an initial cell density of 2 × 105/mL. At 48 hours of culture, supernatants were collected and concentrated 10-fold by ultrafiltration. The TPO assay was performed in triplicate in all cell lines examined. The data show TPO concentrations in each 10-fold concentrated supernatant. The lower limit of detection for TPO was 0.2 fmol/mL.
Abbreviation: ND, not done.
One hundred units per milliliter of IL-1α (4 ng/mL) was added at the initiation of culture.