Approximate IC50 Values of Peptides Inhibiting the Interaction of PfEMP1 or Infected Erythrocytes With CD36 or ICAM-1
| Peptide . | IC50(μmol/L) . | ||
|---|---|---|---|
| Binding of PfEMP1 . | Binding of rC1-2 . | PE Adherence . | |
| Binding to CD36 | |||
| CD36 62-75 | >250 | ND | ND |
| CD36 139-155 | 5.9 | 0.6 | >100* |
| CD36 145-171 | ND | <0.1 | 86 |
| CD36 146-164 | ND | <0.1 | >100* |
| CD36 156-184 | ND | <0.01 | 24.5 |
| CD36 233-250 | 205.9 | ND | ND |
| Binding to ICAM-1 | |||
| ICAM-1 15-20 | 208.3 | ND | 79 |
| Peptide . | IC50(μmol/L) . | ||
|---|---|---|---|
| Binding of PfEMP1 . | Binding of rC1-2 . | PE Adherence . | |
| Binding to CD36 | |||
| CD36 62-75 | >250 | ND | ND |
| CD36 139-155 | 5.9 | 0.6 | >100* |
| CD36 145-171 | ND | <0.1 | 86 |
| CD36 146-164 | ND | <0.1 | >100* |
| CD36 156-184 | ND | <0.01 | 24.5 |
| CD36 233-250 | 205.9 | ND | ND |
| Binding to ICAM-1 | |||
| ICAM-1 15-20 | 208.3 | ND | 79 |
Results summarize the approximate IC50 values of peptide inhibition on the binding of soluble 125I-PfEMP1 to immobilized CD36, binding of CD36 to immobilized rC1-2 [1-179] and inhibition of PE adhesion. Binding of 125I-PfEMP1 to CD36 was measured with SDS extract of the MC strain. Binding of125I-PfEMP1 to ICAM-1 was measured with SDS extract of the ItG-ICAM strain. Blockade of PE adherence was tested with strain A4ultra.
Abbreviation: ND, not determined.
No inhibition at the highest concentration tested.