Comparing the Frequency of Apoptosis and the Level of Bcl-2 Expression in WAS and Normal Lymphocyte Populations
| Incubation Time After Isolation . | Mean Apoptotic Fraction of Lymphocytes* . | Mean Bcl-2 Expression in Lymphocytes† . | ||
|---|---|---|---|---|
| WAS . | Normal . | WAS . | Normal . | |
| None | 0.014 ± 0.013 | 0.0014 ± 0.0012 | 25.3 ± 17.9 | 54.9 ± 28.3 |
| 24 h | 0.17 ± 0.078 | 0.048 ± 0.018 | 34.5 ± 15.2 | 59.7 ± 19.8 |
| 48 h | 0.27 ± 0.18 | 0.10 ± 0.035 | 33.8 ± 10.1 | 62.0 ± 25.4 |
| 96 h | 0.37 ± 0.15 | 0.12 ± 0.044 | ND | ND |
| Incubation Time After Isolation . | Mean Apoptotic Fraction of Lymphocytes* . | Mean Bcl-2 Expression in Lymphocytes† . | ||
|---|---|---|---|---|
| WAS . | Normal . | WAS . | Normal . | |
| None | 0.014 ± 0.013 | 0.0014 ± 0.0012 | 25.3 ± 17.9 | 54.9 ± 28.3 |
| 24 h | 0.17 ± 0.078 | 0.048 ± 0.018 | 34.5 ± 15.2 | 59.7 ± 19.8 |
| 48 h | 0.27 ± 0.18 | 0.10 ± 0.035 | 33.8 ± 10.1 | 62.0 ± 25.4 |
| 96 h | 0.37 ± 0.15 | 0.12 ± 0.044 | ND | ND |
WAS indicates the mean value for a group of 5 WAS patients; normal indicates the mean value for a group of 8 normal controls (5 adults and 3 children). Patient no. 1 was analyzed once, patient no. 2 was analyzed 3 times, and all others were analyzed twice in independent experiments with unbalanced numbers of replicates.
Abbreviation: ND, not determined.
Mean apoptotic fraction of PBL was measured using flow cytometry to quantify cells marked by TUNEL.
Mean Bcl-2 expression in lymphocytes was determined by measuring the MFI of cells stained with fluorescein-conjugated secondary antibody directed against bound antihuman Bcl-2 monoclonal antibody.