Table 1.
Exon/Intron Organization of the mIIb Gene
| Splice Donor | Intron No. (size) | Splice Acceptor |
|---|---|---|
| acatggaagGTGAGCGCTAAAGGACATATGGGCG | 1 (1,623 bp) | GTGCTCATCCAGCTTTCCTACACAGcgtgtccat |
| H G S | V S I | |
| tcgacctcaGTGAGTCTCAAGAATGAAGGGGAAA | 2 (89 bp) | CTCCACCTTTACTTGTGCCCTCCAGgggatgaga |
| F D L | R D E | |
| gtcattgtgGTGGGTACTGTGGACAAGTCAAAGG | 3 (97 bp) | CTACTCTCCTGTGGGTCTGCTCTAGgcctgtgcc |
| V I V | A C A | |
| agagttttcGTAAGCTCTGGTTTGCCATTCGCTC | 4 (222 bp) | GCTGATCCCCGCTTGCCTCTTGTAGgcggagaca |
| E S F | R G D | |
| gtgacccagAAGTCACAGGCAAAAGCAAACAA | 5 (535 bp) | TTCACGACCAGGACTTCTTTTTCAGgctggggag |
| V T Q | A G E | |
| tttttttagGTAGTGCCCAGGAATCCAACCCACT | 6 (91 bp) | AGCCCTCTCCCTGTGCCCTTCCTAGgtctcctgg |
| F F L | G L L | |
| gttaccgggGTAACGCTGGCAGCTCCTTTCCAAG | 7 (312 bp) | CGGTAAACTGTGCTGTGTCTTTCAGgatattcgg |
| G Y R | G Y S | |
| gcactacagAAGGAATGCAGGGGGCGAGGGGG | 8 (135 bp) | ATTTGCTGACCCTTGCTCTCCCCAGagtacgtat |
| S T T | E Y V | |
| ttgggagcgGTGAGTAGTGGCTCTCTCACCGCTT | 9 (160 bp) | CATTTTGGTGTGTCCCTCCATGCAGgtggaaatt |
| L G A | V E I | |
| GgagaacagGTCGGGGCGGTGACCCCATGGGCAT | 10 (142 bp) | AATGGAAGTGCTATTCCTCTTGTAGatggcttca |
| G E Q | M A S | |
| cggggacggGTGAGAAGAGGGAATGTCCCACCCT | 11 (153 bp) | CTAACCTTACCCATCTGGTCCACAGgaggcatga |
| G D G | R H D | |
| gctataatgGTGAGTGGGGGAGCTGCATTTGGCC | 12 (3,121 bp) | CCACACTCTTTCTCTCTCCTTCTAGatattgctg |
| G Y N | D I A | |
| gatacccagGTGACTATGGGTTACAGCCAGCCAG | 13 (273 bp) | TTGACCCTTCTCCCTATGCCTATAGacctgattg |
| G Y P | D L I | |
| tgtgtacagGTGAGCTCTGACTAGGGGGAGGGAC | 14 (84 bp) | AGCGAGCCTCTTCCAATCCCATCAGagctcagcc |
| V Y R | A Q P | |
| agtcagctgGTGAGGAGGTGGAGGTCACGGACTT | 15 (84 bp) | CACAAACCTGCCCTCATCTTTGCAGcttcaacat |
| V S C | F N I | |
| agaagctgcGTGAGTGCCATGGAGTGAGGGGTTG | 16 (89 bp) | TGGATCCTTCCTGCTTGCCATGCAGatctaaagg |
| Q K L | H L K | |
| ttccttcggGTATGCTCAGGCTAGGATGGGAGGG | 17 (107 bp) | CAGACTCTTGCCGTGCGCACGCTAGgatgaggcc |
| F L R | D E A | |
| caggagcagGTAGGAATAGTGGGACGAGCAAGAT | 18 (310 bp) | ACACGCATCCCATTGTGTCCCCTAGacacggatc |
| Q E Q | T R I | |
| agctactgcGTGAGAGAGTCTTTCACTCTACCCA | 19 (117 bp) | CCTAAACCAAGCACTCCCCATACAGgggggactc |
| A T A | G D S | |
| aacattgagGTGAGGTACCACCATGGGGCATAGC | 20 (1,438 bp) | GCCCCACCTGTCTGTGCATCTCCAGggctttgag |
| N I E | G F E | |
| gacacccggGTAAGGGCTCTGTGATGTAACTCTA | 21 (149 bp) | TGACGTTGGATTTTCCCTCTTCCAGataggaatc |
| D T R | I G I | |
| ggtcaggagGTACTGAGCTGGGCAGCATGGCTGA | 22 (260 bp) | CTTCTTTTCACCTTCTCCCCTCCAGcaagaacag |
| V R S | K N S | |
| gcttcgaggGTGAGAGACCAAGCATGGGACGGGG | 23 (212 bp) | TCTCTGGGGACTTGGACACTTGCAGgaattcctt |
| L R G | N S F | |
| acctatgagGTAGGGAGGAGCCTCTGGGTAAGAT | 24 (141 bp) | GCCACCCCGCTATCTCCACCCCCAGctccacaac |
| T Y E | L H N | |
| tctcccaagGTAAGGTTCTGGGAGAGAGAAGGAG | 25 (100 bp) | TTGATTGTGTCCTTGTTCCCCCCAGgtggactgg |
| S P K | V D W | |
| gttctggtgGTGAGAAGGCTCAGCGGGCTCGAGC | 26 (630 bp) | CTTACCACACATCCATCCCCCTCAGagctgcgac |
| V L V | S C D | |
| ctccgccagGTGGGGCTAGACCCGGATGGGCGGG | 27 (229 bp) | CGCCTGTCACCCCCGCCCCATACAGaggccgcag |
| L R Q | R P Q | |
| caagctcggGTAAGAGACCCTGGTTCTCCTGCTG | 28 (218 bp) | GAAGTGACATCTAGTTTGCCCTCAGgtgcagaca |
| Q A R | V Q T | |
| atgtggaagGTGAGGCTGAAAGGGAGACACAAAC | 29 (2,050 bp) | AGCTCCTGTGCCCTTCCCCCTCCAGgctggcttc |
| M W K | A G F | |
Gaagag CAGCAGAGGGGGCGGGGTTCCTGGT | 〈〈〈103 bp〉〉〉 | ACC GAGCTTGACAGTGAT |
| E E ter | poly A signal |
| Splice Donor | Intron No. (size) | Splice Acceptor |
|---|---|---|
| acatggaagGTGAGCGCTAAAGGACATATGGGCG | 1 (1,623 bp) | GTGCTCATCCAGCTTTCCTACACAGcgtgtccat |
| H G S | V S I | |
| tcgacctcaGTGAGTCTCAAGAATGAAGGGGAAA | 2 (89 bp) | CTCCACCTTTACTTGTGCCCTCCAGgggatgaga |
| F D L | R D E | |
| gtcattgtgGTGGGTACTGTGGACAAGTCAAAGG | 3 (97 bp) | CTACTCTCCTGTGGGTCTGCTCTAGgcctgtgcc |
| V I V | A C A | |
| agagttttcGTAAGCTCTGGTTTGCCATTCGCTC | 4 (222 bp) | GCTGATCCCCGCTTGCCTCTTGTAGgcggagaca |
| E S F | R G D | |
| gtgacccagAAGTCACAGGCAAAAGCAAACAA | 5 (535 bp) | TTCACGACCAGGACTTCTTTTTCAGgctggggag |
| V T Q | A G E | |
| tttttttagGTAGTGCCCAGGAATCCAACCCACT | 6 (91 bp) | AGCCCTCTCCCTGTGCCCTTCCTAGgtctcctgg |
| F F L | G L L | |
| gttaccgggGTAACGCTGGCAGCTCCTTTCCAAG | 7 (312 bp) | CGGTAAACTGTGCTGTGTCTTTCAGgatattcgg |
| G Y R | G Y S | |
| gcactacagAAGGAATGCAGGGGGCGAGGGGG | 8 (135 bp) | ATTTGCTGACCCTTGCTCTCCCCAGagtacgtat |
| S T T | E Y V | |
| ttgggagcgGTGAGTAGTGGCTCTCTCACCGCTT | 9 (160 bp) | CATTTTGGTGTGTCCCTCCATGCAGgtggaaatt |
| L G A | V E I | |
| GgagaacagGTCGGGGCGGTGACCCCATGGGCAT | 10 (142 bp) | AATGGAAGTGCTATTCCTCTTGTAGatggcttca |
| G E Q | M A S | |
| cggggacggGTGAGAAGAGGGAATGTCCCACCCT | 11 (153 bp) | CTAACCTTACCCATCTGGTCCACAGgaggcatga |
| G D G | R H D | |
| gctataatgGTGAGTGGGGGAGCTGCATTTGGCC | 12 (3,121 bp) | CCACACTCTTTCTCTCTCCTTCTAGatattgctg |
| G Y N | D I A | |
| gatacccagGTGACTATGGGTTACAGCCAGCCAG | 13 (273 bp) | TTGACCCTTCTCCCTATGCCTATAGacctgattg |
| G Y P | D L I | |
| tgtgtacagGTGAGCTCTGACTAGGGGGAGGGAC | 14 (84 bp) | AGCGAGCCTCTTCCAATCCCATCAGagctcagcc |
| V Y R | A Q P | |
| agtcagctgGTGAGGAGGTGGAGGTCACGGACTT | 15 (84 bp) | CACAAACCTGCCCTCATCTTTGCAGcttcaacat |
| V S C | F N I | |
| agaagctgcGTGAGTGCCATGGAGTGAGGGGTTG | 16 (89 bp) | TGGATCCTTCCTGCTTGCCATGCAGatctaaagg |
| Q K L | H L K | |
| ttccttcggGTATGCTCAGGCTAGGATGGGAGGG | 17 (107 bp) | CAGACTCTTGCCGTGCGCACGCTAGgatgaggcc |
| F L R | D E A | |
| caggagcagGTAGGAATAGTGGGACGAGCAAGAT | 18 (310 bp) | ACACGCATCCCATTGTGTCCCCTAGacacggatc |
| Q E Q | T R I | |
| agctactgcGTGAGAGAGTCTTTCACTCTACCCA | 19 (117 bp) | CCTAAACCAAGCACTCCCCATACAGgggggactc |
| A T A | G D S | |
| aacattgagGTGAGGTACCACCATGGGGCATAGC | 20 (1,438 bp) | GCCCCACCTGTCTGTGCATCTCCAGggctttgag |
| N I E | G F E | |
| gacacccggGTAAGGGCTCTGTGATGTAACTCTA | 21 (149 bp) | TGACGTTGGATTTTCCCTCTTCCAGataggaatc |
| D T R | I G I | |
| ggtcaggagGTACTGAGCTGGGCAGCATGGCTGA | 22 (260 bp) | CTTCTTTTCACCTTCTCCCCTCCAGcaagaacag |
| V R S | K N S | |
| gcttcgaggGTGAGAGACCAAGCATGGGACGGGG | 23 (212 bp) | TCTCTGGGGACTTGGACACTTGCAGgaattcctt |
| L R G | N S F | |
| acctatgagGTAGGGAGGAGCCTCTGGGTAAGAT | 24 (141 bp) | GCCACCCCGCTATCTCCACCCCCAGctccacaac |
| T Y E | L H N | |
| tctcccaagGTAAGGTTCTGGGAGAGAGAAGGAG | 25 (100 bp) | TTGATTGTGTCCTTGTTCCCCCCAGgtggactgg |
| S P K | V D W | |
| gttctggtgGTGAGAAGGCTCAGCGGGCTCGAGC | 26 (630 bp) | CTTACCACACATCCATCCCCCTCAGagctgcgac |
| V L V | S C D | |
| ctccgccagGTGGGGCTAGACCCGGATGGGCGGG | 27 (229 bp) | CGCCTGTCACCCCCGCCCCATACAGaggccgcag |
| L R Q | R P Q | |
| caagctcggGTAAGAGACCCTGGTTCTCCTGCTG | 28 (218 bp) | GAAGTGACATCTAGTTTGCCCTCAGgtgcagaca |
| Q A R | V Q T | |
| atgtggaagGTGAGGCTGAAAGGGAGACACAAAC | 29 (2,050 bp) | AGCTCCTGTGCCCTTCCCCCTCCAGgctggcttc |
| M W K | A G F | |
| GaagagCAGCAGAGGGGGCGGGGTTCCTGGT | 〈〈〈103 bp〉〉〉 | ACCGAGCTTGACAGTGAT |
| E E ter | poly A signal |
Genomic organization at the exon/intron borders are shown beginning with exon 1’s splice donor region. 9 bp of the exons and 25 bp of the introns are shown at the splicing junctions. Total intron size is indicated. Splice donors and acceptors are underlined. The “GC” instead of “GT” splice donors for exons 5 and 8 are double-underlined. The termination codon and polyadenylation signal in exon 30 are boxed, and the left out sequence between the sequences shown is indicated.