Induction of Skin Vasculitis by Injection of FcR+ Mast Cells Into FcRγ −/− Mice
| Inoculation . | FcRγ . | 6-19 . | No. . | Cell Transfer . | Ear Lesions . |
|---|---|---|---|---|---|
| IV | −/− | + | 4 | Macrophage* | 0/4 |
| 5 | LPS-macrophage† | 0/5 | |||
| 9 | BMMC‡ | 5/9 | |||
| −/− | − | 3 | Macrophage | 0/3 | |
| 3 | BMMC | 0/3 | |||
| SC | −/− | + | 6 | Macrophage | 0/6 |
| 6 | LPS-macrophage | 0/6 | |||
| 8 | BMMC | 6/8 | |||
| −/− | − | 3 | Macrophage | 0/3 | |
| 3 | LPS-macrophage | 0/3 | |||
| 3 | BMMC | 0/3 |
| Inoculation . | FcRγ . | 6-19 . | No. . | Cell Transfer . | Ear Lesions . |
|---|---|---|---|---|---|
| IV | −/− | + | 4 | Macrophage* | 0/4 |
| 5 | LPS-macrophage† | 0/5 | |||
| 9 | BMMC‡ | 5/9 | |||
| −/− | − | 3 | Macrophage | 0/3 | |
| 3 | BMMC | 0/3 | |||
| SC | −/− | + | 6 | Macrophage | 0/6 |
| 6 | LPS-macrophage | 0/6 | |||
| 8 | BMMC | 6/8 | |||
| −/− | − | 3 | Macrophage | 0/3 | |
| 3 | LPS-macrophage | 0/3 | |||
| 3 | BMMC | 0/3 |
FcRγ −/− mice were inoculated with 6-19 hybridoma cells as described in Table 1. Seven days later, various FcR+ cells were IV or SC transferred into 6-19–treated FcRγ −/− mice.
Adherent populations from the thioglycolate-induced peritoneal exudate cells were used as macrophages.
Peritoneal macrophages as in the asterisk note were activated with LPS.
Bone marrow cells were cultured in the presence of IL-3 and used after 4 weeks of culture as BMMC.