Population Expansion of hGM R,βc Cells and hIL-3 R,βc Cells Continuously Cultured in the Presence of 10 ng/mL hGM-CSF and hIL-3, Respectively
| Days in Culture . | hIL-3 Rα,βc Cells . | hGM Rα,βc Cells . |
|---|---|---|
| 2 | 4.5 ± 1.5 | 1.1 ± 0.1 |
| 7 | 546 ± 25* | 4.3 ± 0.5 |
| Days in Culture . | hIL-3 Rα,βc Cells . | hGM Rα,βc Cells . |
|---|---|---|
| 2 | 4.5 ± 1.5 | 1.1 ± 0.1 |
| 7 | 546 ± 25* | 4.3 ± 0.5 |
Cells were seeded at an initial cell number of 5 × 104/mL and cell counts are presented after 2 and 7 days in culture. Data are expressed as fold increase in cell number. Results are the mean of three experiments ± SEM. Similar results were obtained for at least 2 clones of each transfect.
Cells were subcultured to 5 × 104/mL at day 4 to avoid overgrowth and death of the cultures.