Specific Primers Used for PCR Amplification of Members of the Ras Family
| Gene . | Sense Primer . | Antisense Primer . | Annealing Tm . | Product Size (bp) . |
|---|---|---|---|---|
| M-Ras | 5′-GGCCTGACTACCAGAAACATG-3′ | 5′-TGCCCAGGGCTTCAGGCTG-3′ | 58 | 645 |
| H-Ras | 5′-TCATTGATGGGGAGACATGTC-3′ | 5′-GTCCTGGGCCTGCCGAGACTC-3′ | 62 | 262 |
| N-Ras | 5′-TGATTGATGGTGAGACCTGC-3′ | 5′-CAGTTCGTGGGCTTGCTTTG-3′ | 60 | 265 |
| R-Ras | 5′-ACTGTGGACGGCATCCCTGC-3′ | 5′-GAAAGAGGAGGCTTCAGACCG-3′ | 60 | 269 |
| β-Actin | 5′-ATGGATGACGATATCGCTGC-3′ | 5′-GCTGGAAGGTGGACAGTGAG-3′ | 60 | 471 |
| Gene . | Sense Primer . | Antisense Primer . | Annealing Tm . | Product Size (bp) . |
|---|---|---|---|---|
| M-Ras | 5′-GGCCTGACTACCAGAAACATG-3′ | 5′-TGCCCAGGGCTTCAGGCTG-3′ | 58 | 645 |
| H-Ras | 5′-TCATTGATGGGGAGACATGTC-3′ | 5′-GTCCTGGGCCTGCCGAGACTC-3′ | 62 | 262 |
| N-Ras | 5′-TGATTGATGGTGAGACCTGC-3′ | 5′-CAGTTCGTGGGCTTGCTTTG-3′ | 60 | 265 |
| R-Ras | 5′-ACTGTGGACGGCATCCCTGC-3′ | 5′-GAAAGAGGAGGCTTCAGACCG-3′ | 60 | 269 |
| β-Actin | 5′-ATGGATGACGATATCGCTGC-3′ | 5′-GCTGGAAGGTGGACAGTGAG-3′ | 60 | 471 |