Phenotypic Characterization of Day 17 Progeny of FL + IL-7 and KL + FL + IL-7–Stimulated Lin−/loSca1+c-kit+ Cells
| Antigen . | % Expression . | |
|---|---|---|
| FL + IL-7 . | KL + FL + IL-7 . | |
| B220 | 82 (7) | 81 (9) |
| CD19 | 49 (13) | 67 (11) |
| CD24 | 99 (0) | 94 (4) |
| CD43 | 89 (8) | 78 (9) |
| cIgM | 0 (0) | 0 (0) |
| c-kit | 89 (4) | 93 (3) |
| flt3 | 0 (0) | 0 (0) |
| Gr1/Mac1 | 0 (0) | 4 (2) |
| Antigen . | % Expression . | |
|---|---|---|
| FL + IL-7 . | KL + FL + IL-7 . | |
| B220 | 82 (7) | 81 (9) |
| CD19 | 49 (13) | 67 (11) |
| CD24 | 99 (0) | 94 (4) |
| CD43 | 89 (8) | 78 (9) |
| cIgM | 0 (0) | 0 (0) |
| c-kit | 89 (4) | 93 (3) |
| flt3 | 0 (0) | 0 (0) |
| Gr1/Mac1 | 0 (0) | 4 (2) |
Lin−/loSca1+c-kit+ cells were cultured in serum-depleted medium supplemented with FL + IL-7 or KL + FL + IL-7 for 17 days, at which time cells were analyzed for the expression of the indicated antigens. Data represent the mean (±SEM) from 3 individual experiments.