FL + IL-7–Responsive Cells Are Exclusively Recruited From the Lin−/loSca1+c-kit+Stem Cell Population
| Cell Phenotype . | Percent of Total Lin−/low Population* . | Cell Production From 500 Starting Cells (×1,000) . | |||
|---|---|---|---|---|---|
| Lin . | Sca1 . | c-kit . | Exp 1 . | Exp 2 . | |
| −/lo | + | + | 2.0 (1.1) | 413 | 172 |
| −/lo | + | − | 4.1 (1.2) | 0 | 0 |
| −/lo | − | + | 14.2 (6.7) | 6 | 0 |
| −/lo | − | − | 79.7 (43.5) | 0 | 0 |
| Cell Phenotype . | Percent of Total Lin−/low Population* . | Cell Production From 500 Starting Cells (×1,000) . | |||
|---|---|---|---|---|---|
| Lin . | Sca1 . | c-kit . | Exp 1 . | Exp 2 . | |
| −/lo | + | + | 2.0 (1.1) | 413 | 172 |
| −/lo | + | − | 4.1 (1.2) | 0 | 0 |
| −/lo | − | + | 14.2 (6.7) | 6 | 0 |
| −/lo | − | − | 79.7 (43.5) | 0 | 0 |
In 2 separate experiments lineage-depleted BM cells were sorted into 4 phenotypically distinct subpopulations, which each were investigated for their ability to respond to FL + IL-7. The total cell production from each subpopulation was established after 12 days of culture and presented as number of cells produced from 500 starting cells.
The percentage of the entire population is shown, whereas the size of each of the sorted populations is shown within the parenthesis.