Single Lin−/loSca1+c-kit+ Cells Undergoing Lymphoid-Restricted Development in Response to FL + IL-7 Have a Combined Myeloid and Lymphoid Differentiation Potential
| . | Cloning Frequency . | Clones Containing Myeloid Cells . | Clones Containing ProB Cells . |
|---|---|---|---|
| Myeloid cocktail* | 95(2)% | 100(0)% | ND |
| FL + IL-7 | 42(1)% | ND | 63(4)% |
| . | Cloning Frequency . | Clones Containing Myeloid Cells . | Clones Containing ProB Cells . |
|---|---|---|---|
| Myeloid cocktail* | 95(2)% | 100(0)% | ND |
| FL + IL-7 | 42(1)% | ND | 63(4)% |
Lin−/loSca1+c-kit+ cells were plated individually in Terasaki plates, and the presence of a single cell per well was verified by microscopy less than 12 hours after initiation of culture to exclude wells containing no or more than 1 cell. After 12 days of culture, the cultures were assayed for the presence of clones (≥3 cells) and the presence of myeloid cells (macrophages and/or granulocytes) by Giemsa-staining. After a total of 15 to 25 days, colonies were analyzed by flow cytometry to investigate the presence of B220+CD19+ proB cells.
Abbreviation: ND, not determined.
Myeloid cocktail = KL + FL + IL-3 + G-CSF + GM-CSF + MGDF + Epo used at predetermined optimal concentrations. The data represent the mean (±SEM) from 4 individual experiments.