PCR Analysis of Micromanipulated Single HRS Cells
| Case . | HRS Cells . | B Cells . | Buffer Controls . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cells . | PCR Products . | Cells . | PCR Products . | Aliquots . | PCR Products . | |||||||
| E4 . | E5/6 . | E7/8 . | E4 . | E5/6 . | E7/8 . | E4 . | E5/6 . | E7/8 . | ||||
| 1 | 10 | 8 | 8 | 8 | 3 | 3 | 3 | 3 | 3 | — | — | — |
| 2 | 10 | 8 | 8 | 8 | 3 | 1 | 1 | 1 | 3 | — | — | — |
| 3 | 10 | 7 | 7 | 6 | 2 | 1 | 1 | 1 | 4 | — | — | — |
| 4 | 12 | 11 | 9 | 8 | 10 | 4 | 4 | 4 | 15 | 1 | 1 | 1 |
| 5 | 12 | 9 | 10 | 8 | 10 | 6 | 5 | 6 | 10 | — | — | — |
| 6 | 12 | 6 | 5 | 6 | 10 | 5 | 4 | 5 | 10 | — | — | — |
| 7 | 12 | 4 | 4 | 4 | 10 | 6 | 5 | 4 | 10 | — | — | — |
| 8 | 16 | 7 | 7 | 3 | 10 | 2 | 2 | 2 | 10 | — | — | — |
| Case . | HRS Cells . | B Cells . | Buffer Controls . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cells . | PCR Products . | Cells . | PCR Products . | Aliquots . | PCR Products . | |||||||
| E4 . | E5/6 . | E7/8 . | E4 . | E5/6 . | E7/8 . | E4 . | E5/6 . | E7/8 . | ||||
| 1 | 10 | 8 | 8 | 8 | 3 | 3 | 3 | 3 | 3 | — | — | — |
| 2 | 10 | 8 | 8 | 8 | 3 | 1 | 1 | 1 | 3 | — | — | — |
| 3 | 10 | 7 | 7 | 6 | 2 | 1 | 1 | 1 | 4 | — | — | — |
| 4 | 12 | 11 | 9 | 8 | 10 | 4 | 4 | 4 | 15 | 1 | 1 | 1 |
| 5 | 12 | 9 | 10 | 8 | 10 | 6 | 5 | 6 | 10 | — | — | — |
| 6 | 12 | 6 | 5 | 6 | 10 | 5 | 4 | 5 | 10 | — | — | — |
| 7 | 12 | 4 | 4 | 4 | 10 | 6 | 5 | 4 | 10 | — | — | — |
| 8 | 16 | 7 | 7 | 3 | 10 | 2 | 2 | 2 | 10 | — | — | — |
Abbreviations: E4, exon 4 of the p53 gene; E5/6, exons 5 and 6; E7/8, exons 7 and 8.