Table 3.

Validation Experiment: Detection of p53 Point Mutations in Single Micromanipulated CD30+ CO Cells

Cell Exon 5 (codon 175) Position 13203 Exon 8 (codon 282) Position 14513
3-150CD30+ 
  1  A/G  T/C  
  2  A/G T/C  
  4  A/G  T/C  
  7  A/G  T/C  
  8 A  T/C  
  9  A/G  T/C  
 10  A  
3-151CD20+ 
 36  G  C  
 38  G  
 39 G  C  
 40  G  C  
 41  G  C  
Wild-type p533-152 G  C  
Cell line CO  A  
Cell Exon 5 (codon 175) Position 13203 Exon 8 (codon 282) Position 14513
3-150CD30+ 
  1  A/G  T/C  
  2  A/G T/C  
  4  A/G  T/C  
  7  A/G  T/C  
  8 A  T/C  
  9  A/G  T/C  
 10  A  
3-151CD20+ 
 36  G  C  
 38  G  
 39 G  C  
 40  G  C  
 41  G  C  
Wild-type p533-152 G  C  
Cell line CO  A  
F3-150

CO cells.

F3-151

B cells isolated as controls.

F3-152

Accession no. X54156.

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