Anticoagulant and ELISA Activities of Affinity-Purified Anti–β2-GPI Antibody Preparations
| . | Normal IgG . | Affinity-Purified aβ2-GPI . | |||||
|---|---|---|---|---|---|---|---|
| No. 1 . | No. 2 . | No. 3 . | No. 4 . | No. 5 . | No. 6 . | ||
| Protein concentration (μg/mL) | 100 | 46 | 41 | 51 | 30 | 54 | 50 |
| dRVVT (seconds prolongation over buffer) | 0.0 | 6.7 | 3.7 | 4.2 | 3.9 | 8.3 | 4.9 |
| Anti–β2-GPI IgG ELISA (OD405) | 0.035 | 3.492 | 3.056 | 3.026 | 3.121 | 2.587 | 3.320 |
| . | Normal IgG . | Affinity-Purified aβ2-GPI . | |||||
|---|---|---|---|---|---|---|---|
| No. 1 . | No. 2 . | No. 3 . | No. 4 . | No. 5 . | No. 6 . | ||
| Protein concentration (μg/mL) | 100 | 46 | 41 | 51 | 30 | 54 | 50 |
| dRVVT (seconds prolongation over buffer) | 0.0 | 6.7 | 3.7 | 4.2 | 3.9 | 8.3 | 4.9 |
| Anti–β2-GPI IgG ELISA (OD405) | 0.035 | 3.492 | 3.056 | 3.026 | 3.121 | 2.587 | 3.320 |