Percentages of Apoptotic, Necrotic, and Living L1210 Cells, After 72 Hours’ Pretreatment With Either a Control Solvent or RA at the Indicated Concentrations, Followed by a 24-Hour PSI or Control Treatment
| Cells . | Apoptotic . | Necrotic . | Living . |
|---|---|---|---|
| Control | 12.0 | 4.5 | 83.5 |
| RA 5 μmol/L | 4.2 | 9.0 | 86.8 |
| RA 50 μmol/L | 23.6 | 11.9 | 64.5 |
| PSI 500 nmol/L | 27.2 | 60.6 | 12.2 |
| PSI 500 nmol/L RA 5 μmol/L | 22.0 | 75.2 | 2.8 |
| PSI 500 nmol/L RA 50 μmol/L | 20.6 | 58.0 | 21.4 |
| Cells . | Apoptotic . | Necrotic . | Living . |
|---|---|---|---|
| Control | 12.0 | 4.5 | 83.5 |
| RA 5 μmol/L | 4.2 | 9.0 | 86.8 |
| RA 50 μmol/L | 23.6 | 11.9 | 64.5 |
| PSI 500 nmol/L | 27.2 | 60.6 | 12.2 |
| PSI 500 nmol/L RA 5 μmol/L | 22.0 | 75.2 | 2.8 |
| PSI 500 nmol/L RA 50 μmol/L | 20.6 | 58.0 | 21.4 |
Data were obtained in each group for 10,000 cells separated from other events based on light scatter characteristics. Apoptotic, necrotic, and living cells were discriminated with the use of CELLQuest v.1.2 software system.