Phenotypes Present in FTOC Started With Limited Numbers of CD34+CD38− CB Cells
| Cytokine Mix . | Days of Culture . | 10 Cells* . | 50 Cells . | ||
|---|---|---|---|---|---|
| CD3+TCRαβ+ † . | CD4+CD8β+ ‡ . | CD3+TCRαβ+ . | CD4+CD8β+ . | ||
| TPO + FL | 3 | 0.0 (0/7) | 0.0 (0/7) | 0.0 (0/3) | 0.0 (0/3) |
| 10 | 0.0 (0/5) | 0.0 (0/5) | 0.0 (0/3) | 0.0 (0/3) | |
| 17 | 0.1 (1/8) | 0.1 (1/8) | 0.0 (0/3) | 0.0 (0/3) | |
| TPO + FL + SCF | 3 | 0.1 (1/7) | 0.1 (1/7) | 0.0 (0/3) | 0.0 (0/3) |
| 10 | 0.3 (2/6) | 0.8 (5/6) | 0.7 (2/3) | 1.0 (3/3) | |
| 17 | 0.3 (2/8) | 0.8 (6/8) | 0.7 (2/3) | 0.7 (2/3) | |
| IL-3 + SCF | 3 | 0.0 (0/7) | 0.0 (0/7) | 0.0 (0/2) | 0.0 (0/2) |
| 10 | 0.0 (0/5) | 0.0 (0/5) | 1.0 (3/3) | 1.0 (3/3) | |
| 17 | 0.1 (1/8) | 0.3 (2/8) | 0.0 (0/3) | 0.3 (1/3) | |
| IL-3 + SCF + IL-6 | 3 | 0.0 (0/7) | 0.0 (0/7) | 0.0 (0/3) | 0.0 (0/3) |
| 10 | 0.0 (0/6) | 0.0 (0/6) | 0.3 (1/3) | 0.7 (2/3) | |
| 17 | 0.0 (0/8) | 0.3 (2/8) | 0.3 (1/3) | 0.7 (2/3) | |
| Cytokine Mix . | Days of Culture . | 10 Cells* . | 50 Cells . | ||
|---|---|---|---|---|---|
| CD3+TCRαβ+ † . | CD4+CD8β+ ‡ . | CD3+TCRαβ+ . | CD4+CD8β+ . | ||
| TPO + FL | 3 | 0.0 (0/7) | 0.0 (0/7) | 0.0 (0/3) | 0.0 (0/3) |
| 10 | 0.0 (0/5) | 0.0 (0/5) | 0.0 (0/3) | 0.0 (0/3) | |
| 17 | 0.1 (1/8) | 0.1 (1/8) | 0.0 (0/3) | 0.0 (0/3) | |
| TPO + FL + SCF | 3 | 0.1 (1/7) | 0.1 (1/7) | 0.0 (0/3) | 0.0 (0/3) |
| 10 | 0.3 (2/6) | 0.8 (5/6) | 0.7 (2/3) | 1.0 (3/3) | |
| 17 | 0.3 (2/8) | 0.8 (6/8) | 0.7 (2/3) | 0.7 (2/3) | |
| IL-3 + SCF | 3 | 0.0 (0/7) | 0.0 (0/7) | 0.0 (0/2) | 0.0 (0/2) |
| 10 | 0.0 (0/5) | 0.0 (0/5) | 1.0 (3/3) | 1.0 (3/3) | |
| 17 | 0.1 (1/8) | 0.3 (2/8) | 0.0 (0/3) | 0.3 (1/3) | |
| IL-3 + SCF + IL-6 | 3 | 0.0 (0/7) | 0.0 (0/7) | 0.0 (0/3) | 0.0 (0/3) |
| 10 | 0.0 (0/6) | 0.0 (0/6) | 0.3 (1/3) | 0.7 (2/3) | |
| 17 | 0.0 (0/8) | 0.3 (2/8) | 0.3 (1/3) | 0.7 (2/3) | |
Table shows fraction of thymic lobes containing indicated phenotypes in FTOC, initiated with limited numbers of CD34+CD38− CB cells that were cultured in medium supplemented with the cytokine mix and for the number of days as indicated. Between parentheses: raw data used to calculate fraction (absolute number over total number of lobes analyzed). Data shown are from 1 representative experiment of 3.
Equivalent number of cells present at the start of the culture.
At least 0.5% of thymocytes express both CD3 and TCRαβ.
At least 0.5% of thymocytes express both CD4 and CD8β.