Phenotypes Present in FTOC Started With Cultured CD34+CD38+ and CD34+CD38− CB Cells
| Cytokine Mix . | Days of Culture . | CD34+CD38+ . | CD34+CD38− . | ||
|---|---|---|---|---|---|
| CD3+ * . | CD4+CD8β+ † . | CD3+ . | CD4+CD8β+ . | ||
| TPO + FL | 3 | 0.9 (9/10) | 0.7 (8/11) | 0.7 (4/6) | 0.8 (6/8) |
| 10 | 0.8 (3/4) | 0.8 (3/4) | 0.6 (3/5) | 0.7 (4/6) | |
| 17 | 0.7 (2/3) | 0.7 (2/3) | 1.0 (5/5) | 1.0 (6/6) | |
| TPO + FL + SCF | 3 | 0.8 (9/11) | 0.8 (9/11) | 1.0 (9/9) | 0.9 (9/10) |
| 10 | 0.6 (2/3) | 0.3 (1/4) | 0.6 (3/5) | 0.7 (4/6) | |
| 17 | 0.6 (3/5) | 0.7 (2/3) | 1.0 (6/6) | 0.8 (3/4) | |
| IL-3 + SCF | 3 | 0.9 (7/8) | 0.7 (7/10) | 1.0 (7/7) | 0.9 (7/8) |
| 10 | 0.5 (3/6) | 0.5 (3/6) | 1.0 (4/4) | 0.8 (3/4) | |
| 17 | 0.6 (3/5) | 0.5 (2/4) | 1.0 (3/3) | 0.5 (3/6) | |
| IL-3 + SCF + IL-6 | 3 | 1.0 (5/5) | 0.8 (4/5) | 1.0 (4/4) | 1.0 (4/4) |
| 10 | 0.8 (5/6) | 0.8 (5/6) | 1.0 (2/2) | 1.0 (2/2) | |
| 17 | 0.8 (3/4) | 0.4 (2/5) | 1.0 (4/4) | 1.0 (4/4) | |
| Cytokine Mix . | Days of Culture . | CD34+CD38+ . | CD34+CD38− . | ||
|---|---|---|---|---|---|
| CD3+ * . | CD4+CD8β+ † . | CD3+ . | CD4+CD8β+ . | ||
| TPO + FL | 3 | 0.9 (9/10) | 0.7 (8/11) | 0.7 (4/6) | 0.8 (6/8) |
| 10 | 0.8 (3/4) | 0.8 (3/4) | 0.6 (3/5) | 0.7 (4/6) | |
| 17 | 0.7 (2/3) | 0.7 (2/3) | 1.0 (5/5) | 1.0 (6/6) | |
| TPO + FL + SCF | 3 | 0.8 (9/11) | 0.8 (9/11) | 1.0 (9/9) | 0.9 (9/10) |
| 10 | 0.6 (2/3) | 0.3 (1/4) | 0.6 (3/5) | 0.7 (4/6) | |
| 17 | 0.6 (3/5) | 0.7 (2/3) | 1.0 (6/6) | 0.8 (3/4) | |
| IL-3 + SCF | 3 | 0.9 (7/8) | 0.7 (7/10) | 1.0 (7/7) | 0.9 (7/8) |
| 10 | 0.5 (3/6) | 0.5 (3/6) | 1.0 (4/4) | 0.8 (3/4) | |
| 17 | 0.6 (3/5) | 0.5 (2/4) | 1.0 (3/3) | 0.5 (3/6) | |
| IL-3 + SCF + IL-6 | 3 | 1.0 (5/5) | 0.8 (4/5) | 1.0 (4/4) | 1.0 (4/4) |
| 10 | 0.8 (5/6) | 0.8 (5/6) | 1.0 (2/2) | 1.0 (2/2) | |
| 17 | 0.8 (3/4) | 0.4 (2/5) | 1.0 (4/4) | 1.0 (4/4) | |
Table shows fraction of CB donors generating indicated phenotypes in FTOC, initiated with CD34+CD38+ or CD34+CD38− CB cells that were cultured in medium supplemented with the cytokine mix and for the number of days as indicated. Between parentheses: raw data used to calculate fraction (absolute number over total number of donors tested).
At least 2% of thymocytes express CD3 (after exclusion of TCRγδ+ cells).
At least 2% of thymocytes express both CD4 and CD8β.