Effect of Added Inhibitors on the Proliferation of Cord Blood–Derived Primitive Human CFC in Standard LTC
| Fresh Medium . | Inhibitor . | % Kill . | |
|---|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | ||
| − | — | 1 ± 4 | 3 ± 2 |
| + | — | 43 ± 5 | 50 ± 4 |
| + | TGF-β1 | 0 ± 5 | 1 ± 3 |
| + | MCP-1 | 4 ± 5 | 2 ± 2 |
| + | MIP-1α | 2 ± 7 | 3 ± 3 |
| Fresh Medium . | Inhibitor . | % Kill . | |
|---|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | ||
| − | — | 1 ± 4 | 3 ± 2 |
| + | — | 43 ± 5 | 50 ± 4 |
| + | TGF-β1 | 0 ± 5 | 1 ± 3 |
| + | MCP-1 | 4 ± 5 | 2 ± 2 |
| + | MIP-1α | 2 ± 7 | 3 ± 3 |
TGF-β1 at 5 ng/mL, MIP-1α and MCP-1 at 100 ng/mL. The effect of TGF-β1, MCP-1, or MIP-1α addition was significant (P < .01) when compared with no addition.
Abbreviations: BFU-E, burst-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte/macrophage.