Distribution of Clonogenic Cells in SortedSCLlacZ/w E12.5 Fetal Liver Fractions
| Stimulus* . | Colony Type . | Progenitor Yield† . | Colony Distribution (%)‡ . | ||
|---|---|---|---|---|---|
| Unsorted . | Sorted . | lacZlow . | lacZhigh . | ||
| Epo | d2 CFU-E | 547 ± 310 | 519 ± 234 | 9 ± 5 | 91 ± 5 |
| Epo/IL 3 | d7 BFU-E | 11 ± 7 | 28 ± 10 | 9 ± 7 | 91 ± 7 |
| d7 myeloid | 23 ± 9 | 46 ± 17 | 20 ± 6 | 80 ± 6 | |
| Stimulus* . | Colony Type . | Progenitor Yield† . | Colony Distribution (%)‡ . | ||
|---|---|---|---|---|---|
| Unsorted . | Sorted . | lacZlow . | lacZhigh . | ||
| Epo | d2 CFU-E | 547 ± 310 | 519 ± 234 | 9 ± 5 | 91 ± 5 |
| Epo/IL 3 | d7 BFU-E | 11 ± 7 | 28 ± 10 | 9 ± 7 | 91 ± 7 |
| d7 myeloid | 23 ± 9 | 46 ± 17 | 20 ± 6 | 80 ± 6 | |
Sorted and unsorted fetal liver cells from 10SCLlacZ/w E12.5 embryos from 3 litters were cultured in duplicate in methylcellulose in the presence of growth factors for 2 or 7 days and the colonies counted. Epo was used at 4 U/mL and IL-3 at 1,000 U/mL.
The number of CFCs in cultures of 1 × 104 unsorted fetal liver cells compared with the calculated yield of CFCs from the sorted fractions. The progenitor cell yield was estimated by summing the number of progenitor cells recovered in the lacZhighand lacZlow fractions for each colony type. The number of progenitor cells was derived by multiplying the progenitor frequency by the % cells in each fraction. Mean values for progenitor cell frequency and the percentage of cells in each fraction are shown in Fig8B. Values given represent the mean ± SD of 10 fetal livers.
The distribution of CFCs between the sorted fetal liver fractions given as a percentage.