Wild-Type Activity of EE372 in Supporting CFUe Formation From Adult Erythroid Progenitor Cells
| Mouse . | CFUe Formation, % of Epo-Induced Colonies . | ||
|---|---|---|---|
| No Epo, No EGF . | Epo . | EGF . | |
| Nontransgenic no. 1 | 0 | 100 | 0 |
| Nontransgenic no. 2 | 0 | 100 | 0 |
| EE372 no. 45 | 0 | 100 | 100 |
| EE372 no. 161 | 0 | 100 | 103 |
| EE372 no. 178 | 0 | 100 | 111 |
| EE372 no. 194* | 0 | 100 | 89 |
| EE372 no. 181† | 10 | 100 | 80 |
| Mouse . | CFUe Formation, % of Epo-Induced Colonies . | ||
|---|---|---|---|
| No Epo, No EGF . | Epo . | EGF . | |
| Nontransgenic no. 1 | 0 | 100 | 0 |
| Nontransgenic no. 2 | 0 | 100 | 0 |
| EE372 no. 45 | 0 | 100 | 100 |
| EE372 no. 161 | 0 | 100 | 103 |
| EE372 no. 178 | 0 | 100 | 111 |
| EE372 no. 194* | 0 | 100 | 89 |
| EE372 no. 181† | 10 | 100 | 80 |
The ability of splenic erythroid progenitor cells from EE372 and nontransgenic control mice to form hemoglobinized colonies was assayed in the presence of either Epo (5 U/mL), hEGF (20 ng/mL), or no cytokines. Benzidine-positive colonies (CFUe) were scored at 48 hours of culture. Values are the means of duplicate samples expressed as the percentage of colonies induced in the Epo treatment groups. The mean values (± standard error) of Epo-induced colonies for mice no. 1, 2, 45, 161, 178, 194, and 181 are 10.5 ± 3.5, 34 ± 3.0, 13 ± 2, 19.5 ± 0.5, 28 ± 6.0, 8.7 ± 1.7, and 25 ± 13.7, respectively (n = 2 for all samples except no. 194, where n = 3).
Bone marrow preparation of erythroid progenitor cells.
hEGF included at 4 ng/mL.