Anchorage-Independent Growth of K-19+Cells in Soft Agar
| . | No. of Colonies (5 × 102 cells/mL) . | No. of Colonies (5 × 103 cells/mL) . |
|---|---|---|
| Neo-control | 4 ± 1 | 27 ± 3 |
| K-Δ 19 | 3 ± 1 | ND |
| K-19+ #8 | 2 ± 0* | 13 ± 1 |
| K-19++ #3 | 0† | 5 ± 2 |
| K-19++ #11 | 0 | ND |
| . | No. of Colonies (5 × 102 cells/mL) . | No. of Colonies (5 × 103 cells/mL) . |
|---|---|---|
| Neo-control | 4 ± 1 | 27 ± 3 |
| K-Δ 19 | 3 ± 1 | ND |
| K-19+ #8 | 2 ± 0* | 13 ± 1 |
| K-19++ #3 | 0† | 5 ± 2 |
| K-19++ #11 | 0 | ND |
Cells were plated in 6-well plates of soft agar and the number of colonies was determined after 2 weeks of culture. The mean values ± SD from triplicate experiments are shown.
Abbreviation: ND, not determined.
Two small colonies appeared in only 1 well.
A very small colony of not more than 4 cells appeared in only 1 of triplicate wells.