Mouse Antivaccinia MoAbs Used for FACS Analysis of Recombinant Vaccinia Virus Infection of DCs and B-LCLs
| Clone . | Isotype . | Reaction With . |
|---|---|---|
| VV1-4G9 | IgG2a | Vaccinia hemagglutinin (cell surface, late) |
| VV4-2F6 | IgG2a | Reacts with D8L, a virion-surface protein |
| VV1-6B6 | IgG2a | Reacts with an early protein of ∼29 kD |
| Clone . | Isotype . | Reaction With . |
|---|---|---|
| VV1-4G9 | IgG2a | Vaccinia hemagglutinin (cell surface, late) |
| VV4-2F6 | IgG2a | Reacts with D8L, a virion-surface protein |
| VV1-6B6 | IgG2a | Reacts with an early protein of ∼29 kD |
MoAbs from mouse ascites, at a concentration of 2 mg/mL or greater, were used at a 1:10,000 dilution, which was optimal.