Table 2. Mitogenic Response of PBMCs and... | American Society of Hematology

Table 2.

Mitogenic Response of PBMCs and T Cells After EBV Stimulation

	PBMCs		T Cells
	Medium	Anti-CD3 + IL-2	Medium	Anti-CD3 + IL-2
Donor 1
Mock	2,921 ± 269	70,411 ± 4,877	1,085 ± 100	57,323 ± 1,326
EBV	6,375 ± 1,922	57,008 ± 4,715	670 ± 158	60,026 ± 1,638
% Mitogen suppression^*	0	19	38	0
Donor 2
Mock	1,990 ± 540	52,263 ± 14,074	2,369 ± 122	62,703 ± 6,290
EBV	1,840 ± 368	22,270 ± 3,772	1,437 ± 241	70,737 ± 3,408
% Mitogen suppression	7.5	57	39	0
Donor 3
Mock	1,760 ± 544	48,836 ± 14,414	8,976 ± 2,453	87,952 ± 2,706
EBV	1,640 ± 234	23,917 ± 1,429	2,532 ± 487	98,977 ± 1,060
% Mitogen suppression	6.8	51	71	0

	PBMCs		T Cells
	Medium	Anti-CD3 + IL-2	Medium	Anti-CD3 + IL-2
Donor 1
Mock	2,921 ± 269	70,411 ± 4,877	1,085 ± 100	57,323 ± 1,326
EBV	6,375 ± 1,922	57,008 ± 4,715	670 ± 158	60,026 ± 1,638
% Mitogen suppression^*	0	19	38	0
Donor 2
Mock	1,990 ± 540	52,263 ± 14,074	2,369 ± 122	62,703 ± 6,290
EBV	1,840 ± 368	22,270 ± 3,772	1,437 ± 241	70,737 ± 3,408
% Mitogen suppression	7.5	57	39	0
Donor 3
Mock	1,760 ± 544	48,836 ± 14,414	8,976 ± 2,453	87,952 ± 2,706
EBV	1,640 ± 234	23,917 ± 1,429	2,532 ± 487	98,977 ± 1,060
% Mitogen suppression	6.8	51	71	0

PBMCs from healthy blood donors were seeded at 1 × 10⁶ cells/mL and stimulated for 24 hours with purified Akata virus or mock-virus preparations (1:10 vol/vol). Aliquots of viable PBMCs or T cells, as measured by trypan blue exclusion, were seeded at 1.5 × 10⁵ cells in 200 μL of medium containing anti-CD3 MoAb OKT3 (1:200) and IL-2 (50 U/mL). Cells were grown for an additional 72 hours. During the final 18 hours, cells were pulsed with [³H]-thymidine (1 μCi/well). Results are expressed as the mean ± SD from triplicate wells.

Negative suppression values were given a value of 0.

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