Mitogenic Response of T Cells After EBV Stimulation and Anti-CD95 Treatment
| . | Medium . | IL-2 . | Anti-CD3 . | Anti-CD3 + IL-2 . |
|---|---|---|---|---|
| Donor 1 | ||||
| Mock | 1,117 ± 248 | 16,142 ± 404 | 37,643 ± 5,012 | 96,345 ± 3,067 |
| EBV | 1,543 ± 434 | 3,351 ± 277 | 1,175 ± 60 | 11,107 ± 2,419 |
| % Mitogenic suppression* | 0 | 79 | 70 | 88 |
| Donor 2 | ||||
| Mock | 198 ± 20 | 8,821 ± 272 | 10,348 ± 2,955 | 74,696 ± 2,997 |
| EBV | 795 ± 401 | 2,052 ± 100 | 8,769 ± 364 | 32,894 ± 1,688 |
| % Mitogenic stimulation | 0 | 77 | 15 | 56 |
| Donor 3 | ||||
| Mock | 86 ± 39 | 11,835 ± 2,488 | 18,947 ± 1,270 | 64,983 ± 2,963 |
| EBV | 119 ± 27 | 775 ± 160 | 11,105 ± 605 | 9,110 ± 816 |
| % Mitogenic stimulation | 0 | 93 | 41 | 86 |
| . | Medium . | IL-2 . | Anti-CD3 . | Anti-CD3 + IL-2 . |
|---|---|---|---|---|
| Donor 1 | ||||
| Mock | 1,117 ± 248 | 16,142 ± 404 | 37,643 ± 5,012 | 96,345 ± 3,067 |
| EBV | 1,543 ± 434 | 3,351 ± 277 | 1,175 ± 60 | 11,107 ± 2,419 |
| % Mitogenic suppression* | 0 | 79 | 70 | 88 |
| Donor 2 | ||||
| Mock | 198 ± 20 | 8,821 ± 272 | 10,348 ± 2,955 | 74,696 ± 2,997 |
| EBV | 795 ± 401 | 2,052 ± 100 | 8,769 ± 364 | 32,894 ± 1,688 |
| % Mitogenic stimulation | 0 | 77 | 15 | 56 |
| Donor 3 | ||||
| Mock | 86 ± 39 | 11,835 ± 2,488 | 18,947 ± 1,270 | 64,983 ± 2,963 |
| EBV | 119 ± 27 | 775 ± 160 | 11,105 ± 605 | 9,110 ± 816 |
| % Mitogenic stimulation | 0 | 93 | 41 | 86 |
Purified T cells from healthy blood donors were seeded at 2 × 105 cells/well and stimulated for 24 hours with EBV or mock-virus preparations (1:20 vol/vol), followed by the addition of 5 μg/mL anti-CD95 IgM MoAb CH-11. Cells were cultured for an additional 24 hours, after which the anti-CD3 MoAb OKT3 (1:200) and/or IL-2 (50 U/mL) was added. Cells were cultured for an additional 72 hours. During the final 18 hours, cells were pulsed with [3H]-thymidine (1 μCi/well). Results are expressed as the mean ± SD from triplicate wells.
Negative suppression values were given a value of 0.