Loss of Clonogenic Potential After Ceramide Treatment
| Cells . | Pretreatment3-150 . | Concentration . | No. of Colonies (/1,000 cells plated)3-151 . |
|---|---|---|---|
| Daudi | C2-dehydroceramide | 20 μmol/L | 637 ± 41 |
| C2-ceramide | 20 μmol/L | 19 ± 2 | |
| Raji | C2-dehydroceramide | 20 μmol/L | 799 ± 61 |
| C2-ceramide | 20 μmol/L | 38 ± 9 |
| Cells . | Pretreatment3-150 . | Concentration . | No. of Colonies (/1,000 cells plated)3-151 . |
|---|---|---|---|
| Daudi | C2-dehydroceramide | 20 μmol/L | 637 ± 41 |
| C2-ceramide | 20 μmol/L | 19 ± 2 | |
| Raji | C2-dehydroceramide | 20 μmol/L | 799 ± 61 |
| C2-ceramide | 20 μmol/L | 38 ± 9 |
Cells were cultured in the presence of the indicated concentration of cell-permeable ceramide. After 48 hours of culture, cells were washed twice with 2% FCS/RPMI1640 and trypan blue-unstained viable cells (1,000 cells/mL) were plated in a semisolid culture medium.
Data are expressed as the mean ± SD of triplicate cultures.